肝细胞癌基因组DNA拷贝数变异的性别差异性  

Sex-dependent differences in DNA copy number alterations in hepatocellular carcinoma

作　　者：闻炳基[1] 丛文铭[2] 王爱忠[3] 贺松琴[1] JIANG Hong-mei 董辉[2] HOU Li-fang 朱忠政[1] 

机构地区：[1]解放军113医院肿瘤科,宁波315040 [2]第二军医大学东方肝胆外科医院病理科,上海200438 [3]解放军113医院病理科,宁波315040 [4]Department of Statistics, Northwestern University, Evanston, IL 60208, USA [5]Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611,USA

出　　处：《第二军医大学学报》2012年第1期5-9,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30470791);南京军区医学科技创新基金(08MA023,09MA043);宁波市自然科学基金(2009A610126)~~

摘　　要：目的比较男性与女性肝细胞癌(HCC)在基因组DNA拷贝数变异(CNA)方面的差异性。方法采用高分辨率微阵列比较基因组杂交技术(array-CGH)检测17例女性与46例男性HCC患者的CNA差异。结果女性HCC染色体片段1q21.3-q22扩增频率(76.5%vs 37.0%,P=0.009)、11q11扩增频率(35.3%vs 0.0%,P=0.000 2)、19q13.31-q13.32扩增频率(23.5%vs 0.0%,P=0.004)和16p11.2丢失频率(35.3%vs 6.5%,P=0.009)显著高于男性,而男性则有更高频率的11q11丢失(63.0%vs 17.6%,P=0.002)。进一步统计分析结果显示,11q11扩增与19q13.31-q13.32扩增(P=0.042)及16p11.2丢失(P=0.033)显著相关,而1q21.3-q22扩增与19q13.31-q13.32扩增(P=0.046)显著相关。结论 CNA在性别特异性HCC演进过程中可能发挥重要作用。Objective To identify the sex-related DNA copy number alterations （CNA） in hepatocellular carcinoma （HCC）. Methods High-resolution array comparative genomic hybridization（array-CGH） was used to examine 17 female and 46 male HCCs. Two-tailed Fisher＇s exact test or X^2 test was used to compare the differences in CNA between females and males. Results The overall frequencies and patterns of CNA in female and male cases were similar. However, female HCC tumors presented more copy number gains compared to male on 1@1.3-q22（76.5% vs 37.00/00, P=0. 009）, 11q11（35.3M vs 0.0%, P=0. 000 2） and 19q13.31-q13.32（23.5M vs 0.0%, P=0. 004）, and more loss on 16p11.2（35.3% vs 6.5%, P=0. 009）. Relative to females, male cases had more copy number loss on 11q11（63.0% vs 17.6%, P=0. 002）. Further analyses showed that 11qll gain was correlated with 19q3.31-q13.32 gain（P=0. 042） and 16pll. 2 loss（P=0. 033）, while lq21.3-q22 gain was correlated with 19q13.31-q13.32 gain（P=0. 046）. Conclusion Our findings suggest that CNA may play an important rolein sex-related difference in HCC development.

关 键 词：微阵列比较基因组杂交 拷贝数变异 性别分布 肝肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]