矿物元素和葡萄糖对木聚糖酶活力影响的研究  

Study on Effects of Mineral Elements and Glucose on Xylanase Activity

作　　者：李淑丽[1] 王娇[1] 尹清强[1] 常娟[1] 左瑞雨[1] 郑秋红[1] 王平 王潇 刘俊熙 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省饲料微生物工程技术研究中心,河南周口466000

出　　处：《江西农业学报》2012年第1期106-108,111,共4页Acta Agriculturae Jiangxi

基　　金：河南省郑州市科技创新团队项目(112PCXTD339)

摘　　要：为探讨硫酸铵、氯化钙、磷酸氢二钾、植酸钙镁和葡萄糖对米曲霉产木聚糖酶能力的影响,采用5因素3水平的正交设计方法对其进行了分析。结果表明:矿物元素和葡萄糖对木聚糖酶活力均有一定的影响,其影响顺序为:硫酸铵>磷酸氢二钾>葡萄糖>植酸钙镁>氯化钙,最优组合的添加比例分别为1.00%、0.40%、0.00%、0.00%、0.00%。其最高酶活出现在固体培养的72 h,木聚糖酶活力达到537.56 U/g(P<0.05)。To determine the effects of ammonium sulfate,calcium chloride,dipotassium hydrogen phosphate,phytin and glucose on xylanase production of Aspergillus oryzae,the orthogonal design（5 factors× 3 levels） was used in this study.The results showed that some mineral elements and glucose could affect xylanase production,the order of their influence was ammonium sulfate dipotassium hydrogen phosphate glucose phytin calcium chloride,and their optimum addition rates were 1.00%,0.40%,0.00%,0.00% and 0.00%,respectively.The highest xylanase activity was 537.56 U/g at the 72nd hour after solid-state fermentation（P0.05）.

关 键 词：米曲霉 木聚糖酶 矿物元素 葡萄糖 

分 类 号：Q556.2[生物学—生物化学]