产γ-PGA菌种选育及固态培养条件的优化  

Screening a Strain Producingγ-Polyglutamic Acid and Optimization of Solid-state Fermentation

作　　者：蒋洪梅[1] 武明月[1] 徐良[1] 徐速[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《酿酒》2012年第1期94-98,共5页Liquor Making

基　　金：东北农业大学开放性实验资助;编号10600125c

摘　　要：从纳豆中分离筛选一株产γ-PGA菌株SK-1,以大豆为培养基固态发酵生产γ-聚谷氨酸,紫外、红外光谱扫描所提产物,结果与对照标准品图谱基本一致。通过正交试验对固体培养条件进行优化,得到γ-PGA固态发酵最佳条件:温度35℃,时间24h,装填量50g/250mL,接种量5%.产物提取条件试验表明:添加不同比例乙醇对γ-PGA产量基本无影响,提取时无需调节浸提液pH。A strain SK-1 producing poly-γ-glutamic acid was isolated from the natto. Using soybeans as culture, the solid-state fermentation product was confirmed by ultraviolet spectrophotometry and Fourier infrared spectroscopy asγ -PGA. Through the orthogonal experiments, the optimum conditions of solid-state fermentation were： temperature 35℃ , fermentation time 24h, soybean weight 50g/250mL and inoculation amount 5%.The tests of extraction conditions showed： different proportions of ethanol hardly has effect on extraction ratios and γ -PGA was extracted without adjusting pH.

关 键 词：Γ-PGA 菌种筛选 产物鉴定 固态培养优化 

分 类 号：TQ226.36[化学工程—有机化工] TS262.15[轻工技术与工程—发酵工程]