堆肥中高温纤维素酶菌株的筛选及其酶性质研究  

Screening and enzyme properties of thermophilic cellulose-decomposing bacteria from composting

作　　者：薛桥丽[1] 王炜[2] 胡永金[1] 杜丽娟 金铁铃[1] 

机构地区：[1]云南农业大学,云南昆明650201 [2]安徽文王酿酒股份有限公司,安徽阜阳236400 [3]云南农业科学院农业质量标准与检测技术研究所,云南昆明650223

出　　处：《中国酿造》2012年第1期30-33,共4页China Brewing

基　　金：国家自然科学基金项目(30960308);云南省自然科学基金项目(2008ZC057M)

摘　　要：从堆肥中分离获得20株耐高温纤维素分解菌,经过复筛,HC-5菌株产酶活性最高。HC-5的生长特性表明,其最适pH值为7.0,最适温度为50℃。菌株HC-5的CMCase和β-glucosidase酶作用活性范围均在40℃～70℃,但最适作用温度分别为60℃和50℃;Avicelase酶作用活性范围在40℃～60℃,而在50℃时酶作用活性最高。HC-5所产的纤维素分解酶具有良好的热稳定性,但随贮温的增加,其热稳定性呈现下降的趋势。Twenty thermophilic cellulose-decomposing bacterial strains were selected from composting.A strain HC-5 was isolated by solid media with Congo red test in secondary screening.The optimal growth temperature and pH value of HC-5 were 50℃ and 7.0,respectively.The activities of CMCase and β-glucosidase of HC-5 could be maintained in the ranges of 40℃～70℃,while their optimal temperature was 60℃ and 50℃,respectively.The activity of HC-5 avicelase could be maintained in the ranges of 40℃～60℃,and its optimal temperature was 50℃.The cellulose-decomposing enzymes produced by HC-5 had good thermal stability,but with the storage temperature increased,the thermal stability presented a downward trend.

关 键 词：堆肥 纤维素分解菌 生物降解 酶活 酶学性质 

分 类 号：Q93-331[生物学—微生物学]