表达淀粉酶、糖化酶葡糖酸醋杆菌工程菌的构建  

Construction of Gene Engineering Gluconacetobacter Expressing Amylase and Glucoamylase

作　　者：潘凌鸿[1] 李欣[1] 颜彩玲[1] 黄建忠[1] 

机构地区：[1]福建师范大学工业微生物教育部工程研究中心生命科学学院福建省现代发酵技术工程研究中心,福建福州350108

出　　处：《微生物学杂志》2011年第6期29-35,共7页Journal of Microbiology

基　　金：福建省科技厅重大项目(2005Q007)

摘　　要：采用基因融合技术,将葡糖酸醋杆菌Gluconacetobacter hansenii ATCC23769分泌蛋白CMCax的信号肽序列分别与来源于枯草芽胞杆菌的淀粉酶基因、黑曲霉的糖化酶基因融合构建融合蛋白,连入能在G.hansenii ATCC23769自主复制的载体pbs-H1S中,电击转入G.hansenii ATCC23769,构建能内源表达淀粉酶、糖化酶,以及淀粉酶-糖化酶的葡糖酸醋杆菌。淀粉平板透明圈检测结果和DNS测酶活结果显示,构建的3种工程菌能成功表达并分泌淀粉酶和糖化酶。A signal peptide of secreted protein CMCax from Gluconacetobacter hansenii ATCC23769 was respectively fused with amylase gene from Bacillus subtilis WB600 and glucoamylase gene from Aspergillus niger to construct fusion proteins using gene fusion technique.The fusion proteins were linked to vector pbs-H1S capable of self-replicate in G.hansenii ATCC23769 and were transformed into G.hansenii ATCC23769 by electric shock to construct engineering Gluconacetobacter strains which could express amylase,glucoamylase and amylase-glucoamylase.The results of starch plate transparent ring test and DNS enzymatic activity test showed that the three engineering strains could express and secret amylase and glucoamylase successfully.

关 键 词：细菌纤维素 葡糖酸醋杆菌 淀粉酶 糖化酶 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]