原生质体电融合选育维生素K_2高产菌株  

Breeding of High-yield Strain of Vitamin K_2 by Protoplast Electro Fusion

作　　者：许静[1] 姚振乐[1] 杨小娇[1] 谢科[1] 刘国庆[1] 

机构地区：[1]合肥工业大学生物与食品学院,合肥230009

出　　处：《食品工业》2012年第2期80-83,共4页The Food Industry

摘　　要：试验采用双亲灭活原生质体电融合选育维生素K2高产菌株,探究了灭活和电融合条件。结果表明:经热灭活和紫外灭活后,在成串脉冲频率2 MHz,成串电压32 V,脉冲融合电压500 V,脉冲宽度40μs,脉冲个数6个的条件下进行电融合,筛选得到一株优良菌株BKU-6,其维生素K2的产量达到39.6 mg/L,远高于产量低的亲本菌株CICC10262。The experiment used parents inactivated protoplast electro fusion to breed High-yield Strain of Vitamin K2,explored the inactivated strains and the electro fusion conditions.The results showed that through thermal inactivated and UV inactivated,and then under the condition of the fusion of frequency 2 MHz,voltage 32 V,fusion voltage 500V,40 μm,pulse number 6,got a good strain BKU-6,the yield of vitamin K2 reached 39.6 mg/L,much higher than the low yield of parents CICC10262 strains.

关 键 词：维生素K2 电融合 热灭活 紫外灭活 

分 类 号：TQ924[轻工技术与工程—发酵工程]