韭菜18S rRNA基因序列分析和分类学意义  

Analysis of Allium tuberosum 18S rRNA gene sequence and significance in taxonomy

作　　者：侯进慧[1] 蔡侃[1] 樊继强[1] 孔文刚[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,江苏徐州221008

出　　处：《食品工业科技》2012年第4期258-260,共3页Science and Technology of Food Industry

基　　金：徐州市科技计划项目(XZZD0924);徐州工程学院大学生创新创业项目;徐州工程学院校级课题(2011)

摘　　要：通过PCR方法扩增韭菜18S rRNA基因,测序获得1664bp的DNA序列,GenBank登录号是JF509958。将韭菜与GenBank中一些物种的18S rRNA基因序列进行序列对比,结果表明,韭菜18S rRNA基因序列与天门冬目的葱科、石蒜科、天门冬科的物种序列相似度高。通过测序绘制出韭菜18S rRNA二级结构。为韭菜资源在分子水平上的研究奠定了基础。Allium tuberosum 18S rRNA gene Sequence were amplified with PCR,and a 1664bp DNA sequence were further analyzed.The accession number of the sequence in Genbank is JF509958.The gene sequence of Allium tuberosum 18S rRNA was analyzed with other species in GenBank.The result showed,Allium tuberosum was related to many families within Asparagales,such as Alliaceae,Amaryllidaceae and Asparagaceae.A 2D Radial drawing of Allium tuberosum 18S rRNA sequence was drawn.Reference data for further research of Allium tuberosum in molecular level was provided.

关 键 词：韭菜 18S RRNA 序列分析 

分 类 号：Q789[生物学—分子生物学]