油茶皂素的提取及纯化新方法  

New study on extraction and purification of tea saponin

作　　者：王金元[1] 费学谦[1] 

机构地区：[1]中国林科院亚热带林业研究所,浙江富阳311400

出　　处：《食品工业科技》2012年第4期274-277,共4页Science and Technology of Food Industry

基　　金："948"引进项目(2009-4-56);国家"十一五"科技支撑课题(2009BADB1B03)

摘　　要：采用乙醇为浸提剂提取油茶籽饼粕中的油茶皂素,正交实验结果表明,油茶皂素提取的较优工艺条件为:乙醇浓度为50%,浸提温度60℃,料液比1:10,浸提时间2h,在此条件下油茶皂素的得率为16.66%,粗产品的油茶皂素的纯度为62.37%。用高速逆流色谱法对粗品进行分离纯化,初步的实验结果表明:乙酸乙酯:正丁醇:水(1:1:2,V/V/V)是适合于油茶皂素分离纯化的溶剂系统,能将油茶皂素含量提高到91.8%。Tea saponin was extracted from seeds of camellia oleifera with alcohol as extraction solvent.The results of orthogonal test showed that the optimum technological conditions were as follows, ethanol concentration 50%, soaking temperature 60℃,ratio of liquid to solid 1：10 and soaking time 2h.Under the conditions, the tea saponin yield was 16.66% ,and its purity in crude product was 62.37%.Then the crude tea saponin was further purificated and separated by HSCCC.The preliminary results showed that the solvent system of ethyl acetate-n-butanol- water（ 1：1：2,V/V/V）was suitable for the purification of tea saponin and the content can be increased to 91.8%.

关 键 词：油茶皂素 提取 高速逆流色谱 纯化 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程]