天麻提取物的制备及其对灰树花发酵的影响  

Preparation of Gastrodia elata BL. extracts and their effects on Grifola frondosa in submerged culture

作　　者：张勇[1] 吴天祥[1] 徐晓宝[2] 王娜[1] 付红伟[3] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学化学与化工学院,贵州贵阳550025 [3]浙江大学生物医学工程与仪器科学学院,浙江杭州310027

出　　处：《食品与机械》2012年第1期150-153,共4页Food and Machinery

基　　金：国家自然科学基金资助项目(编号:31060272)

摘　　要：通过6种不同处理方式制取天麻提取物,用HPLC测定各种提取方法中提取物中的天麻素含量,并在灰树花发酵体系中添加天麻提取物,研究天麻提取物对灰树花细胞生长和多糖生物合成的影响。结果表明,6组天麻提取物中的天麻素含量不同,其中第6组天麻提取物添加浓度在5%(V/V)时能显著促进灰树花的菌体生长、胞外多糖(EPS)和胞内多糖(IPS)的生物合成(P<0.05),并对天麻提取物发酵前后的物质变化做了初步的HPLC图比较。The Gastrodia elata BL.extracts were obtained with six different processing methods,which were added into the fermentation system of Grifola frondosa,and the gastrodia contents in different Gastrodia elata BL.extracts were determined by HPLC.The results showed that the gastrodia contents were different in different Gastrodia elata BL.extracts.Moreover,among them the Gastrodia elata BL.extracts from the No.6 method,which contained 5%（V/V）in a medium,could promote the biomass growth,extracellular polysaccharide（EPS） and intracellular polysaccharide（IPS） biosynthesis of Grifola frondosa significantly（P0.05）,and the HPLC for Gastrodia elata BL.extracts before and after fermentation were compared preliminarily.

关 键 词：天麻提取物 天麻素 灰树花 深层发酵 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]