转人乳铁蛋白基因大米的营养成分及亚慢性毒性研究  

Nutritional components and sub-chronic toxicity of genetically modified rice expressing human lactoferrin

作　　者：胡贻椿[1] 朴建华[1] 杨晓光[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所卫生部微量元素营养重点实验室

出　　处：《卫生研究》2012年第1期6-12,共7页Journal of Hygiene Research

基　　金：国家重点基础研究发展计划(973)项目(No.2007CB109207);国家转基因生物新品种培育科技重大专项(No.2008ZX08011-005,2011ZX08011-005)

摘　　要：目的比较转人乳铁蛋白基因（hLf）大米与其亲本大米在营养成分上的差异,并观察其是否具亚慢性毒性作用。方法对大米中的主要成分及矿物质、维生素进行测定并比较。将初断乳Wistar大鼠按性别、体重随机分为4组饲喂90天,自由进食与饮水。依据大米成分分析结果配制饲料：转hLf大米高剂量饲料（A组,大米添加量为71.45%）、转hLf大米中剂量饲料（B组,大米添加量为35.725%）、亲本大米高剂量饲料（C组,大米添加量为71.01%）和AIN-93G对照饲料（D组）。观察大鼠的体重、进食量、血常规、血生化、脏器系数、骨密度情况,并对大鼠的脏器进行病理学检查。结果转hLf大米与亲本大米在总的主要营养成分、矿物质、维生素上没有显著差异,但在乳铁蛋白及Fe含量上具明显差异。转hLf大米组血常规（WBC、HGB、RBC、MCH）、血生化（AST、Glu）、脏器系数、骨密度的个别指标与亲本对照组或AIN-93对照组存在显著差异（P〈0.05）;其他观察指标与两个对照组均无显著差异。结论部分指标虽然存在组间差异,但仍在正常范围内,未发现转hLf大米对健康具有毒副作用。Objective To compare the nutritional components of genetically modified rice expressing human lactoferrin（hLf） with its parental rice,and to observe the sub-chronic toxicity of hLf rice.Methods The nutritional components of hLf rice and its parental rice were determined by the National Standard Methods.Eighty weanling Wistar rats were randomly divided into 4 groups based on their gender and body weight： group A（hLf rice high-dose group with 71.45% rice）,group B（hLf rice medium-dose group with 35.725% rice）,group C（parental rice group with 71.01% rice） and group D（AIN-93G diet group）,and the latter two groups were used as the control.Body weight,dietary intake,blood routine test,blood biochemical examination,organ coefficient,bone density and the pathology of organs were investigated at the end of a 90-day feeding experiment.Results Except for human lactoferrin and Fe,there was no difference of main nutritional components,minerals and vitamins between groups.The differences of some indicators of blood routine（WBC,HGB,RBC and MCH）,blood biochemistry（AST and GLU）,organ coefficient and bone density between group A and B（hLf rice） with group C（parental rice） or group D（AIN-93G） were significant,while no difference of other indicators.Conclusion Although some differences were observed,all indicators were still in the normal reference range.Therefore,there was no sign of toxic and adverse effects for hLf rice on rats.

关 键 词：转基因大米 乳铁蛋白 营养成分 亚慢性毒性 

分 类 号：R151.3[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]