顺序注射光度法测定铁强化酱油中NaFeEDTA含量  

Determination of NaFeEDTA in iron fortified soy sauce by sequential injection spectrophotometry

作　　者：陈志霞[1] 王夕云[1] 禇宁 范世华[1] 

机构地区：[1]东北大学分析科学研究中心,沈阳110004

出　　处：《分析仪器》2012年第1期27-30,共4页Analytical Instrumentation

摘　　要：在酸性介质中,从NaFeEDTA解离出的Fe3+与KSCN反应,生成的红色络合物在483nm处会产生强吸收。基于这一原理,建立了顺序注射光度法测定强化酱油中NaFeEDTA含量的方法。线性范围5.0～100.0mg/L,检出限(3σ)1.4mg/L,相对标准偏差1.7%(n=11,20.0mg/L),采样频率60/h。回收率在95%～109%之间,实际样品测定结果与推荐方法一致。本法比推荐方法节省试剂99%,并省去醇沉步骤。方法简单,快速,易于自动化,已用于实际样品的测定。In acidic medium,a red complex will be formed in the reaction between KSCN and iron（Ⅲ） dissolved out from NaFeEDTA and it has a special absorption at 483nm.Based on this principle,a sequential injection spectrophotometry method is proposed for the determination of NaFeEDTA contents in iron fortified soy sauce.The linear range of the calibration curve was 5.0~100mg/L with the detection limit（3σ） of 1.4mg/L and the variation coefficient of 1.7%（n=11,20mg/L）.The sample throughput was 60/h.The recoveies were in the range of 95%~109%.The test results are in agreement with those of recommded method.The reagent consumption decreased by 99% and the procedure of pretreatment by alcohols was romoved.The method is simple,fast and easy to be automated,and has been already applied to the determination of NaFeEDTA in practical iron fortified soy sauce samples.

关 键 词：NAFEEDTA 分光光度法 顺序注射 酱油 

分 类 号：TS264.21[轻工技术与工程—发酵工程] O657.3[轻工技术与工程—食品科学与工程]