菊米提取液对人结肠癌SW620细胞增殖和凋亡的影响  

Effect of Jumi Extraction on Proliferation and Apoptosis of Human Colon Cancer Cell Line SW620

作　　者：司马军 陈健[2] 刘丹丹[2] 沈健[2] 王索安[2] 郑鸣之[2] 仇容[2] 

机构地区：[1]浙江省杭州红十字会医院普外科,杭州310004 [2]浙江医学高等专科学校

出　　处：《中国中西医结合外科杂志》2012年第1期39-42,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：浙江省中医药科技计划项目(2010ZB022);浙江省嘉兴市科技计划项目(2010AY1017);浙江省医药卫生科研基金项目(2008A033)

摘　　要：目的:观察菊米提取液对人结肠癌细胞增殖和凋亡的影响。方法:用不同浓度的菊米提取液处理SW620细胞,MTT法检测菊米提取液对SW620细胞增殖的抑制作用;流式细胞术检测细胞凋亡率;比色法测定Caspase-3/7酶和Caspase-6酶活性。结果:菊米提取液对SW620细胞增殖有抑制作用,IC50为0.012mg/mL,且呈一定的量效关系;随着药物浓度的增加(0.001、0.002、0.005、0.01mg/mL),细胞凋亡率分别为(6.46±0.02)%、(9.77±0.01)%、(13.11±0.04)%、(18.47±0.05)%,对照组细胞凋亡发生率为(4.83±0.01)%,显示菊米提取液诱导的细胞凋亡作用随浓度的增大而增加。同时Caspase-3/7酶和Caspase-6酶活性显著增强。结论:菊米提取液在能抑制体外培养的SW620细胞增殖并诱导其凋亡,Caspase-3、6、7活性增强可能是菊米提取液诱导SW620细胞凋亡的机制之一。Objective To explore the effect of Jumi extract on proliferation and apoptosis of human colon cancer cell line SW620.Methods SW620 cells and culture media containing Jumi extract （0.001,0.002,0.005,or 0.01 mg/mL） were co-incubated for 48 h.Cell proliferation rate,cell apoptosis,caspase-3/7 and caspase-6 activity were determined by methyl thiazolyl tetrazolium （MTT） colorimetric assay,flow cytometry analysis and spectrophotometric detection,respectively.Results The proliferation of SW620 cells was significantly inhibited by Jumi extract （0.001-0.01 mg/mL） in a dose-dependent manner.IC50 is 0.012mg/mL.Apoptosis rates in the cells treated with Jumi extract （0.001,0.002,0.005,or 0.01 mg/mL） were （6.46±0.02）%,（9.77±0.01）%,（13.11±0.04）% and （18.47±0.05）% respectively,which were higher than those in the control group （4.83±0.01） %.Compared with the control group,caspase-3/7 and caspase-6 activity were significantly increased in all Jumi treated groups.Conclusion The Jumi extract can inhibit the proliferation of colon cancer cell line SW620 in vitro in a dose-dependent manner and promote cell apoptosis through caspase-3,caspase-7 and caspase-6 pathway.

关 键 词：菊米提取液 结肠癌 增殖 细胞凋亡 

分 类 号：Q95-33[生物学—动物学] R735.35[医药卫生—肿瘤]