茶渣蛋白的提取、有限酶解及抗氧化性质研究  

Study on Extraction,Limited Enzymatic Hydrolysis and Antioxidant Activity of Tea Dregs Protein Complexes

作　　者：赵立娜[1] 汪少芸[1] 饶平凡[1] 江勇 黄家炽 周绍迁 

机构地区：[1]福州大学生物科学与工程学院,福州350002 [2]福建仙洋洋食品科技有限公司,福建宁德352101

出　　处：《中国食品学报》2012年第2期53-60,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省新世纪优秀人才支持计划资助项目(JA10012)

摘　　要：目的:茶渣含有丰富的蛋白质,本研究旨在利用废弃茶渣,开发一种新型、绿色、天然的抗氧化剂。方法:利用碱溶酸沉法提取茶渣蛋白,酶法水解后对茶渣蛋白多肽复合物的体外抗氧化性进行研究,并与传统的合成抗氧化剂VC、BHT进行抗氧化效果的比较。结果:茶渣蛋白提取的最佳工艺为固液比1:40(W/V),提取时间60min,碱浓度0.1mol/L,提取温度90℃,最佳提取次数1次,最佳沉淀pH范围3.0～4.0,在以上条件下茶渣蛋白的提取率达76%;复合蛋白酶水解茶渣蛋白的最佳酶解工艺条件为:酶解温度50℃、最适pH7.0、酶/底物6000U/g、底物质量分数1.5%、水解时间20min;有限酶解所得酶解度为9%。0.1mg/mL多肽复合物DPPH·自由基清除率为90.30%,1.0mg/mL多肽复合物羟自由基(·OH)清除率为65.18%。在亚油酸体系中,多肽复合物的抗氧化性高于维生素C,略低于BHT。结论:茶渣蛋白多肽复合物具有较高的安全性及抗氧化性,有取代合成抗氧化剂的潜力。Objective：There are abundant protein in tea dregs, this study is to develop a new, green, natural an- tioxidants by utilizing waste tea dregs. Methods：Tea dregs protein was extracted by a mixed solution system,then antioxi- dant activity of these proteins in vitro and liposome-meat system after limited enzymatic hydrolysis was, studied, and comparison with the traditional synthetic antioxidants VC, BHT was engaged. Results：the optimum condition of protein extraction was as follows： ratio of material to solvent 1：40, the extraction time 60 min, the NaOH concentration 0.10 mol/L, the extraction temperature 90 ℃, and one time extraction. Under this condition with pH3.0-4.0, the maximumextracting rate of protein complexes was 76%. By the screening of the hydrolysis of TP with complex protease, the tech- nology was selected as temperature 50 ℃, pH 7.0, ratio of protease to substrate 6 000 U/g, substrateconcentration 1.5%, hydrolysis time 20 min. The DH of tea dregs protein after limited hydrolysis was determined to be 9%. When the con- centration of peptide complexes was 0.1 mg/mL, the radical eliminating ratio on DPPH was 90.30%, and the eliminating ratio on hydroxyl radical was 65.18% when the concentration of peptide complex was 1.0 mg/mL. The antioxidant activity was more than VC, but less than BHT in LA system. Conclusion： With high security and strong antioxidant activity, tea dregs protein peptide complexes has the potential to replace synthetic antioxidants.

关 键 词：茶渣蛋白 酶解 抗氧化 

分 类 号：TS272[农业科学—茶叶生产加工]