四种皱皮木瓜挥发性成分的分析  

Analysis of volatile components of four species of Chaenomeles speciosa Nakai

作　　者：徐双双[1,2] 耿岩玲[2] 孙瑜 张恒[1] 王晓[2] 

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]山东省科学院中药过程控制研究中心山东省分析测试中心,山东济南250014 [3]山东省中医药大学药学院,山东济南250355

出　　处：《山东科学》2012年第1期13-18,共6页Shandong Science

基　　金：山东省科技攻关项目(2007GG20009005);济南市高校院所自主创新计划(201004010)

摘　　要：采用顶空固相微萃取-气相色谱质谱联用(HS-SPME-GC-MS)技术分析测定了4个品种皱皮木瓜的挥发性成分。考察了不同萃取头和萃取部位对木瓜挥发性成分萃取效果的影响,选用65μm PDMS/DVB SPME萃取头萃取木瓜中的挥发性成分得到较好的效果,不同部位果皮和果肉对挥发性成分的萃取效果影响不大。应用峰面积归一化法测定各挥发性成分的相对含量,4个品种共鉴定出60种挥发性成分,主要为酯类、烷烃类和烯烃类物质,占总峰面积的90%以上。结果表明,4个品种皱皮木瓜的挥发性成分种类和含量均存在差异。该方法快速准确,为进一步开发利用皱皮木瓜资源提供了参考依据。We employed headspace solid phase microextraction （SPME） and GC-MS to analyze the volatile components of four different species of Chaenomeles speciosa Nakai. We also investigated the effects of different SPME fibers and extraction positions on extraction results. Experimental results show that 65 pm PDMS/DVB fiber exhibits better extraction result. The pericarp and flesh of different positions have not significant influence on their volatile components. We further emloloyed peak area normalization method to determine the relative content of each volatile component. We identified 60 volatile components, which mainly included esters, alkanes and alkenes whose total content exceeded 90%. The varieties and relative contents of the volatile components of these Chaenomeles speciosa Nakai were different. This method is rapid and accurate, so it can provide reference for further development and utilization of Chaenomeles speciosa Nakai resources.

关 键 词：皱皮木瓜 挥发性成分 顶空固相微萃取 气质联用 

分 类 号：O657.63[理学—分析化学]