水果及其制品中果胶含量的比色法测定条件优化  

Study on the colorimetry determination conditions of pectin in fruits and derived products

作　　者：庞荣丽[1] 张巧莲[2] 郭琳琳[1] 方金豹[1] 谢汉忠[1] 李君[1] 罗静[1] 吴丰魁[1] 

机构地区：[1]中国农业科学院郑州果树研究所/农业部果品及苗木质量监督检验测试中心(郑州) [2]许昌职业技术学院园林园艺系

出　　处：《果树学报》2012年第2期302-307,共6页Journal of Fruit Science

基　　金：农业部2006年农业行业标准制修定项目(农财发[2006]64号)

摘　　要：为研究水果及其制品中果胶测定方法,以半乳糖醛酸为标准物质,对硫酸-咔唑比色法测定水果及其制品中果胶含量的比色条件如吸收波长、咔唑-无水乙醇溶液用量、硫酸用量、显色温度、显色时间、显色后稳定时间等进行了优化和系统研究。结果表明,最佳显色条件为在1.0 mL滤液中,加入0.25 mL 0.1%咔唑-无水乙醇溶液,迅速加入5.0 mL硫酸,在85℃水浴中加热20 min,自来水浴中冷却,显色后1.5 h内在525 nm波长条件下测定。在此条件下,标准曲线线性范围广、相关性良好,精密度和准确度高,均符合相关标准要求。In order to research the determination method of pectin content in fruits and derived products, coloring conditions of sulfuric acid-carbazole eolorimetry including absorption wavelength, amount of car- bazole-ethanol solution and sulfuric acid, temperature, reaction time, and stabilization time after coloring were optimized using galaeturonic acid as standard substance. The results showed that the optimum color- ing conditions were adding 0.25 mL 0.1% carbazole-ethanol solution and adding 5.0 mL sulfuric acid quickly to 1.0 mL filtrate, water bathing for 20 min at 85 ~C, cooling down quickly with running tape water bath, and measuring at 525 nm wavelength within 1.5 h after coloring. Under the conditions, it had wide linear range standard curve, good correlation, high precision and accuracy, and were in line with relevant standards.

关 键 词：果胶 比色法 硫酸 咔唑 

分 类 号：S66[农业科学—果树学]