pH条件对短短芽孢杆菌FJAT-0809-GLX次生代谢物产生的影响  

Effects of Initial pH Value for the Fermentation of Brevibacillus brevis FJAT-0809-GLX on the Generation of Secondary Metabolites Substances

作　　者：陈峥[1] 刘波[1] 朱育菁[1] 胡桂萍[1] 车建美[1] 唐建阳[1] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福建农业学报》2012年第1期71-76,共6页Fujian Journal of Agricultural Sciences

基　　金：国家"973"前期项目(2011CB111607);国家"948"项目(2011-G25);福建省青年科技创新基金(2011J05053)

摘　　要：应用GC-MS对10种不同初始pH条件下发酵的FJAT-0809-GLX发酵液的丙酮萃取液进行初步成分鉴定,从这10种发酵液中得到匹配率≥90%成分11种,各发酵液中的成分存在明显差异,其中共有成分1种,为六氢吡咯并[1,2-a]吡嗪-1,4-二酮。在匹配率≥90%成分中,功能性成分按其功能可分为2类,一类为挥发性或芳香类物质,与该发酵液具有的特殊香气有关,包括5-甲基呋喃醛、2-甲基-3-羟基-4-吡喃酮、苯乙醛、棕榈酸、顺式十八碳-9-烯酸、甲基环戊烯醇酮;另一类为防腐或抑菌类成分,包括2-甲基-3-羟基-4-吡喃酮、2-呋喃甲醇、六氢-吡咯[1,2-a]吡嗪-1,4-二酮和5-羟甲基糠醛。初始pH 6～12的发酵液对大肠杆菌K88均有抑制效果,其中初始pH为7的抑菌效果最佳,抑菌圈直径达到20.33mm。不同的初始pH影响着发酵终点pH,进而影响着短短芽孢杆菌FJAT-0809-GLX次生代谢物的产生。Components of FJAT-0809-GLX fermentation liquid in10 different initial pH values were analyzed by GC- MS. Eleven compounds with the match degree above 90 % were identified. The compounds found commonly in these 10 fermentation liquid was Hexahydro-pyrrolo [-1,2-a] pyrazine-1,4-dione. There were two sorts of functional compounds in the FJAT-0809-GLX fermentation liquids; one is volatile compounds which relate to the special aroma of this fermentation liquid, including 5-methyl-2-Furancarboxaldehyde, Maltol, Benzeneacetaldehyde, n-Hexa- decanoic acid, Oleic Acid, and 2-Hydroxy-3-methyl-2-cyclopenten-l-one. The other sort of functional compound had antiseptic and bacteriostatic effect, including Maltol, 2-Furanmethanol and Hexahydro-pyrrolo [-1, 2-a] pyrazine- 1,4-dione and 5-（hydroxymethyl）-2-Furancarboxaldehyde. The fermentation liquid with the initial pH value of 6 to 12 had inhibitory activity against K88. The pH value 7 had the best inhibitory activity; the diameter of inhibition zone was 20.33mm. Different initial pH values led to different terminal pH value, and then affect the generation properties of secondary metabolites of Brevibacillus brevis FJAT-0809-GLX.

关 键 词：次生代谢物 发酵过程 PH 气相色谱质谱联用 短短芽孢杆菌 丙酮萃取 

分 类 号：S432[农业科学—植物病理学]