酶法制备α-环糊精反应体系中癸醇的分离  

Extraction Separation of 1-Decanol in Enzymatic Reaction System for α-Cyclodextrin Production

作　　者：卢滋[1] 李兆丰[2,1] 顾正彪[2,1] 洪雁[2,1] 李才明[1] 班宵逢[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2012年第2期203-206,共4页Food and Fermentation Industries

基　　金：国家自然科学基金(批准号:31101228);江苏省自然科学基金(批准号:BK2011152);霍英东教育基金会高等院校青年教师基金资助

摘　　要：建立了利用液液萃取技术分离酶法制备α-环糊精反应体系中复合剂——癸醇的方法。首先,在α-环糊精溶液中加入癸醇形成α-环糊精-癸醇复合物沉淀后,以该混合体系为研究对象,考察了萃取剂种类及其用量、萃取温度和萃取时间等因素对萃取法分离癸醇的影响,确定了萃取条件:以15倍于癸醇体积的乙醚作为萃取剂,在30℃下萃取30 min。其次,以酶法制备α-环糊精的反应体系为研究对象,在最佳萃取条件下分离癸醇后,所测得的α-、β-、γ-环糊精收率与采用传统方法——水蒸气蒸馏法所得收率相当,进一步验证了萃取法能够将α-环糊精-癸醇复合物中的癸醇有效分离出来。An extraction method was developed to separate the complex reagent 1-decanol in enzymatic reaction system for α-eyelodextrin production. Firstly, a certain amount of 1-decanol was added to α-cyclodextrin solution to form α-cyclodextrin-l-decanol complexes for the parameter study of extraction separation. Effects of type and volume of extraction solvent, extraction time, and extraction temperature on extraction separation of 1-decanol were investiga- ted. The results showed that the optimum conditions were that ethyl ether, 15 times as many as the volume of 1-de- canol, was used as extraction solvent to extract 1-decanol at 30℃ for 30 min. Secondly, under above optimum conditions, 1-decanol in enzymatic reaction system for α-cyclodextrin production was extracted. The results showed that, after extraction separation of 1-decanol, α-,β and γ-cyelodextrin yields determined were almost the same as those by the traditional separation method--steam distillation, which further verified that 1-decanol in the a-cyclodextrin-l-de- canol complexes could be effectively separated using extraction method. Furthermore, compared with steam distil- lation, the proposed extraction method was simple, energy-saving and efficient.

关 键 词：液液萃取 Α-环糊精 癸醇 分离 水蒸气蒸馏 

分 类 号：TQ923[轻工技术与工程—发酵工程]