饲喂益生菌Lactobacillus casei Zhang对高脂日粮大鼠肝脏脂质代谢的作用  

Effect of Lactobacillus casei Zhang Administration on Liver Lipids Metabolism of High-Fat Diet Induced Hypercholesterolemia Rats

作　　者：张勇[1] 杜瑞亭[1] 何秋雯[2] 礼贺[1] 张和平[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院/国家奶牛产业技术体系乳制品研究室/乳品生物技术与工程教育部重点实验室,呼和浩特010018 [2]江南大学食品科学与技术学院,江苏无锡214122

出　　处：《中国农业科学》2012年第5期943-950,共8页Scientia Agricultura Sinica

基　　金：国家高技术研究发展计划项目(2011AA100901;2011AA100902);农业部现代农业产业技术体系建设项目(nycytx-0501)

摘　　要：【目的】研究不同剂量益生菌L.casei Zhang(2.0×1010CFU/d、2.0×109CFU/d和2.0×108CFU/d)及其发酵乳饮料(2.0×108CFU/mL/d)对高胆固醇血症大鼠肝脏脂质的治疗作用。【方法】采用高脂饲料诱导高脂血症,采用相应试剂盒测定肝脏总胆固醇水平(TC)和甘油三酯(TG)水平、粪便总胆汁酸水平与胆固醇水平以及血清载脂蛋白ApoAⅠ与ApoB水平。【结果】益生菌各剂量组和发酵饮料组与高脂模型组相比,肝脏总胆固醇水平(TC)和甘油三酯(TG)水平均显著降低(P<0.05或P<0.01)。各试验组均可显著增加高血脂大鼠粪便总胆汁酸水平和血清载脂蛋白ApoAⅠ水平以及显著降低血清载脂蛋白ApoB水平(P<0.01),但对粪便胆固醇水平影响不明显(P>0.05)。【结论】益生菌L.casei Zhang主要通过增加总胆汁酸的排出和调节载脂蛋白水平来改善高血脂大鼠肝脏脂质水平。【Objective】 The therapeutic effect of different doses of L.casei Zhang(2.0×1010 CFU/d,2.0×109 CFU/d and 2.0×108 CFU/d) and its fermented milk beverage(2.0×108 CFU/mL/d) on liver lipids of hypercholesterolemia rats was investigated.【Method】Hypercholesterolemia rats were induced by high fat feeding.Liver TC and TG levels,fecal total bile acid and cholesterol levels as well as serum ApoAⅠand ApoB levels were dertermined by commercial kits.【Result】 The results showed that liver TC and TG levels were significantly decreased in all probiotic treatment groups compared to hypercholesterolemia model group(P<0.05 or P<0.01).In addition,fecal total bile acid levels and serum ApoAⅠlevels were significantly promoted and serum ApoB levels significantly reduced in all probiotic treatment groups compared to model group,while fecal cholesterol levels were changed with no significance(P>0.05).【Conclusion】 It is suggested that enhancing elimination of bile acids and regulating apolipoprotein levels could be mainly contributed to liver lipids ameliorated by probiotic L.casei Zhang in hypercholesterolemia rats.

关 键 词：LACTOBACILLUS CASEI Zhang 肝脏脂质 高血脂大鼠 总胆汁酸 载脂蛋白 粪便胆固醇 

分 类 号：S816.7[农业科学—饲料科学]