不同微量元素对出芽短梗霉发酵的影响  

Effects of Different Microelements on Aureobasidium pullulans Fermentation

作　　者：韩丛琴[1] 王兴华[2] 

机构地区：[1]山西大学生物技术研究所,山西太原030006 [2]山西大学生命科学学院,山西太原030006

出　　处：《山东农业科学》2012年第3期112-115,共4页Shandong Agricultural Sciences

基　　金：山西省高校科技开发项目(20091002)

摘　　要：以实验室保存的出芽短梗霉UV60为菌株,研究了CrCl3、ZnSO4、MnSO4和C5H11NO2Se在出芽短梗霉液体发酵过程中对短梗霉多糖产量及生物量的影响。经过单因素及正交试验获得了最优的微量元素组成,即0.5 mg/L CrCl3、0.15 g/L ZnSO4、0.2 mg/L MnSO4、15μg/L C5H11NO2Se。采用上述优化的微量元素浓度,多糖产量为28.9 g/L,较优化前多糖产量明显提高,为短梗霉多糖的发酵生产提供了参考依据。The Aureobasidium pullulans UV60 strain conserved in laboratory was used to study the effect of CrCl3, ZnS04, MnS04 and CsHnNO2Se on the yield and biomass of puUulan during Aureobasidium pullu- hms fermentation. The optimal microelement combination was obtained through the single factor test and or- thogonal design, which was 0.5 mg/L CrC13, 0.15 g/L ZnS04, 0.2 mg/L MnS04, 15 μg/L CsHnNO2Se. Under these conditions, the pullulan yield was 28.9 g/L, which was significantly higher than before. It pro- vided a reference for pullulan production.

关 键 词：出芽短梗霉 短梗霉多糖 发酵 微量元素 

分 类 号：TQ925[轻工技术与工程—发酵工程]