硒和锌对人食管癌细胞株Eca109生长增殖影响的血清生理学研究  

Effects of selenium and zinc on the proliferation of human esophageal cancer cell line studied by serophysiology

作　　者：肖慧娟[1] 江岩[2] 齐玉梅[3] 周小乔[1] 龚偲[1] 黄承钰[1] 李鸣[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生学教研室,成都610041 [2]新疆大学生命科学与技术学院 [3]天津市第三中心医院营养科

出　　处：《卫生研究》2012年第2期185-190,共6页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30571559)

摘　　要：目的观察不同剂量硒、锌灌胃大鼠血清对人食管癌细胞株Eca109生长增殖的影响。方法将SD大鼠随机分为7组(基础饲料组、低硒组、高硒组、低锌组、高锌组、低硒低锌组、高硒高锌组),每组8只。喂养30天后取大鼠血清培养人食管癌细胞株Eca109和人正常肝上皮细胞株HL7702。用AAS法分别测定各组大鼠血清硒、锌;采用MTT法、3H-TDR掺入实验研究不同浓度硒、锌灌胃大鼠血清对两株细胞生长增殖的影响。结果 (1)基础饲料组血清硒、锌水平最低,高锌组血清锌最高;高硒高锌灌胃组大鼠血清硒水平最高,低硒低锌灌胃组血清硒水平次之,均明显高于基础饲料组;而此两组大鼠血清锌与基础饲料喂饲组大鼠血清锌水平差异无统计学意义(P>0.05);(2)与小牛血清对照组相比,只有高硒高锌灌胃组大鼠血清从第72h起抑制癌细胞生长(P<0.05),其余各组均促进食管癌细胞的生长;且该组大鼠血清也抑制肝细胞生长(P<0.05);(3)高硒高锌灌胃组大鼠血清明显抑制食管癌细胞DNA合成(P<0.05),其余各组与对照组作用相近,但该组对肝细胞DNA合成的抑制作用也最强。结论硒、锌在吸收、代谢等方面可能存在相互抑制作用;血清硒、锌含量较低会促进人食管癌细胞的增殖,而增加硒、锌的摄入可提高血清硒、锌的含量且可能抑制癌细胞的增殖。Objective The effect of serum from rats supplemented with selenium and zinc on the proliferation of human esophageal cancer cell line Eca109 was observed by serophysiology.Methods Rats were randomly divided into seven groups.Eight rats in each group were fed with basic feeds（deprived of both selenium and zinc）.The experimental rat groups were supplemented with selenium or zinc at low or high dosage intragastrically for 30 days Serum selenium and zinc content of rats was measured by Graphite Furnace Atomic Absorption Spectrometry（GFAAS） and Flame Atomic Absorption Spectrophotometry（FAAS）.MTT assay,3H-TDR incorporation and flow cytometry were used to explore the effect of serum from different rat groups on the growth and proliferation of cancer cell line Eca109 cells.Results（1） The content of serum zinc in the high zinc group was the highest and the content of serum zinc was the lowest in basic diet group.The content of serum selenium in high selenium and high zinc group was the highest and the content of serum selenium was the lowest in the basic diet group.（2） In comparing the growth of control cancer cell group cultured with calf serum,the growth of cancer cells cultured with the serum from high selenium and high zinc rats was inhibited in culturing for 72 h,but the growth of normal liver cells were also inhibited.The growth of cancer cells were promoted by serum from other groups.（3） Both MTT assay and3H-TDR incorporation test showed that the DNA synthesis in cancer cells was inhibited by the serum from high selenium and high zinc group,but the DNA synthesis of normal liver cells was also inhibited by this type of serum.The result of DNA synthesis in other cell groups was closed to the control group.Conclusions Low serum selenium and zinc might promote the growth of EC cell.Elevating the content of serum selenium and zinc by increasing selenium and zinc intake might inhibit EC cell proliferation.

关 键 词：食管癌 硒 锌 细胞增殖 血清生理学 

分 类 号：R735.1[医药卫生—肿瘤] R730.1[医药卫生—临床医学]