L-阿拉伯糖对α-葡萄糖苷酶抑制活性的体外试验研究  

Inhibitory effect of L-arabinose on α-glucosidase tested in vitro

作　　者：姚晓芬[1,2] 王鑫[1] 应茵[1] 王竹[2] 张立实[1] 杨月欣[2] 

机构地区：[1]四川大学华西公共卫生学院,四川成都610041 [2]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《中国食品卫生杂志》2012年第2期102-105,共4页Chinese Journal of Food Hygiene

基　　金：十一五国家科技支撑计划(2006BAD27B01)

摘　　要：目的探讨L-阿拉伯糖对小肠α-葡萄糖苷酶的抑制作用及与阿卡波糖的联用效应。方法利用体外实验,提取大鼠小肠粘膜上清液为α-葡萄糖苷酶粗酶液,分别以终浓度为60 mg/ml蔗糖、20 mg/ml麦芽糖/α-糊精为底物,建立最佳抑制反应体系,测定L-阿拉伯糖对α-葡萄糖苷酶抑制活性(IC50)及抑制作用类型;采用4×3析因设计,研究L-阿拉伯糖与阿卡波糖联用效果。结果以蔗糖、麦芽糖、α-糊精为底物时,L-阿拉伯糖对小肠α-葡萄糖苷酶活性均有一定抑制作用,但选择性较高地抑制蔗糖酶活性,当L-阿拉伯糖添加量为0.5%蔗糖浓度时酶活性抑制百分率>50%,最高酶活性抑制百分率约93%,且有良好剂量-反应关系,IC50为0.164 mg/ml,抑制类型为反竞争性抑制(Ki,0.558 mg/ml);与阿卡波糖联用二者有交互作用,尤其以蔗糖为底物时联用效果较明显,联合应用可能提高了抑制活性。结论 L-阿拉伯糖有抑制α-葡萄糖苷酶活性作用,尤其对蔗糖酶有良好的选择性抑制;在α-葡萄糖苷酶抑制作用方面,其与阿卡波糖有一定联用效果,L-阿拉伯糖在含糖食品中可能有较良好的实际应用前景。Objective To investigate the inhibitory effect of L-arabinose and L-arabinose combined with acarbose on the activities of α-glucosidase in vitro.Methods The homogenate of α-glucosidas prepared by scraping mucosa from small intestine of rats was incubated with sucrose,maltose and α-dextrin（in a final concentration of 60 mg/ml,20 mg/ml and 20 mg/ml respectively） as optimal reaction systems to determine the inhibitory effects of L-arabinose on the activities of α-glucosidase（IC50） and the type of inhibition.Results L-arabinose inhibited the activities of α-glucosidase in a dose-dependent manner,especially selectively inhibiting the activity of sucrase in an uncompetitive manner（Ki,0.558 mg/ml） with an IC50 of 0.164 mg/ml.The combination of L-arabinose with acarbose might enhance the effect of inhibition,especially while using sucrose as substrate.Conclusion L-arabinose inhibited the activities ofα-glucosidase,especially the activities of sucrase.The combination of L-arabinose with acarbose might increase the inhibitory effect.L-Arabinose might have good prospects for practical application in sweet food.

关 键 词：Α-葡萄糖苷酶 Α-葡萄糖苷酶抑制剂 L-阿拉伯糖 降血糖机制 

分 类 号：R96[医药卫生—药理学]