江苏及周边地区副溶血性弧菌分子流行病学特征研究  

Molecular epidemiology traits of Vibrio parahaemolyticus in Jiangsu and surrounding areas

作　　者：刘丽萍[1,2] 陈盛霞[1] 徐岚[2] 李薇薇[3] 裴晓燕[3] 郭云昌[3] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013 [2]镇江市疾病预防控制中心,江苏镇江212000 [3]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2012年第2期106-109,共4页Chinese Journal of Food Hygiene

基　　金：江苏省自然科学基金项目(BK2010350);镇江市科技支撑-社会发展项目(SH2011068)

摘　　要：目的了解江苏及周边地区副溶血性弧菌的分子流行病学特征。方法对2010年江苏及周边地区食物中毒事件中分离的、经API生化鉴定的63株副溶血性弧菌进行毒力基因(tdh、trh)测定、血清学分型和脉冲场凝胶电泳(PFGE)分型。结果 tdh阳性率79.4%(50/63),trh阳性率1.6%(1/63);病人株血清型以O3∶K6为主(26/57),食品株以O1∶KUT为主(4/6);PFGE分型显示A-E群间相似度大于60%。结论江苏及周边地区中不同地区的副溶血性弧菌具有高度同源性,该地区副溶血性弧菌引起的食物中毒可能具有相同的污染源。Objective To understand the molecular epidemiology traits of Vibrio parahaemolyticus in Jiangsu and surrounding areas.Methods Biochemical identification,tdh and trh gene detection,serological typing and pulse-field gel electrophoresis（PFGE） were applied to 63 Vibrio parahaemolyticus strains isolated from food poisoning incidents occurred in Jiangsu and surrounding areas in 2010.Results The positive rates of tdh and trh were 79.4%（50/63） and 1.6%（1/63） respectively.The main serological type of strains isolated from patients was O3∶K6（26/57）,and that from food was O1∶KUT（4/6）.The patterns of PFGE showed that the similarity of groups from A to E was more than 60%.Conclusion Strains isolated from different regions in Jiangsu and surrounding areas were in high homology.The food poisoning incidents occurred in these areas might be contaminated by Vibrio parahaemolyticus strains from the same source.

关 键 词：副溶血性弧菌 血清型 同源性 食源性致病菌 

分 类 号：R378.3[医药卫生—病原生物学] R181.3[医药卫生—基础医学]