Sau-PCR分子分型技术及应用  

Sau-PCR molecular typing technology and its application

作　　者：张淑红[1] 吴清平[1] 徐晓可[1] 张菊梅[1] 郭伟鹏[1] 

机构地区：[1]广东省华南应用微生物重点实验室--省部共建国家重点实验室培育基地,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东省微生物研究所,广东广州510070

出　　处：《中国食品卫生杂志》2012年第2期180-184,共5页Chinese Journal of Food Hygiene

基　　金：国家自然科学基金项目(U1031003);广东省科技计划项目(2010B031000020、2009B030803010)

摘　　要：Sau-PCR是2005年新发展的一种限制性内切酶(Sau3AI)酶切和PCR扩增相结合的分子分型技术,该技术是在扩增片段长度多态性(AFLP)和随机扩增多态性DNA片段(RAPD)方法的基础上建立起来的,具有重复性好、简单、快捷等优点,近年来被逐步应用于食品污染和院内感染的分子流行病学调查。本文对Sau-PCR的原理、技术特点及其应用情况等进行了介绍。Sau-PCR is a novel amplification technique for tracing the genetic fingerprint of microorganisms.Based on the digestion of genomic DNA with restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site,the reproducibility of the assay was as high as amplified fragment length polymorphism（AFLP）and is as quick and easy as random amplified polymorphic DNA（RAPD）.Nowadays,Sau-PCR has been gradually used to determine the source of food pathogens and monitor the epidemiology of nosocomial infection.The principle,technical characteristics and the application of Sau-PCR were introduced in this paper.

关 键 词：Sau-PCR 分子分型 食源性致病菌 应用 

分 类 号：R378[医药卫生—病原生物学]