薄层层析-紫外分光光度法测定银杏茶中银杏酸含量  

DETERMINATION OF GINKGOLIC ACIDS IN GINKGO TEA BY TCL-UV SPECTROPHOTOMETRY

作　　者：秦俊哲[1] 郭云霞[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2012年第1期4-7,共4页Journal of Shaanxi University of Science & Technology

基　　金：陕西省重大科技创新专项项目(2010ZKC11-05)

摘　　要：采用薄层层析-紫外分光光度法建立了一种银杏茶中银杏酸快速检测的方法.采用正己烷索氏提取银杏茶中的银杏酸;以硅胶G为固定相,石油醚∶乙酸乙酯∶冰醋酸(18∶1∶1)为展开剂对银杏酸进行分离纯化;紫外分光光度法测定,波长为307nm.结果表明:该方法测定银杏酸含量的线性关系良好,线性范围为0.009～0.093mg/mL(R2=0.999 7),精密度RSD为2.15%,平均回收率为100.90%,测定结果同HPLC法结果对照无明显差异,适用于银杏茶中银杏酸含量的测定.To establish a fast determination method of ginkgolie acids（GAs） in ginkgo tea by TLC-UV. The sample through hexane Soxhlet extraction and TCL purification which was ac- complished on G plate by using the mobile phase of petroleum ether ： ethyl acetate ： glacial acetic acid （18 ： 1 ： 1）,then determined by UV spectrophotometry at 307 nm. The results showed that there was a linear relationship within the range of 0. 009-0. 093 mg/mL（R^2 = 0. 999 7）, the RSD was 2.15% ,the average recovery was 100.90% ,the results being detec- ted by UV spectrophotometry were close to those by HPLC. The method is suitable for the GAs analysis in ginkgo tea.

关 键 词：银杏茶 银杏酸 薄层层析 紫外分光光度法 

分 类 号：TS201.6[轻工技术与工程—食品科学]