产β-葡萄糖苷酶野生真菌的筛选鉴定及酶学性质研究  

Screening and identification of a wild β-glucosidase producing fungal and its enzymatic property

作　　者：覃拥灵[1] 何海燕[1] 刘园园[1] 杨海芳[1] 黄金群[1] 

机构地区：[1]河池学院化学与生命科学系,广西宜州546300

出　　处：《中国酿造》2012年第3期53-57,共5页China Brewing

基　　金：广西自然科学基金项目(2010GXNSFA013103);广西教育厅重点科研项目(201102ZD032);河池学院重点科研项目(2009YAZ-N003)

摘　　要：从原始森林腐木的土壤中筛选得到1株高产β-葡萄糖苷酶活力菌株Lcxs9,表型分析及ITS rDNA序列分子鉴定为芽枝霉菌,酶活为7.18U/mL,在pH值为4~9范围内,60℃以下酶活力稳定,酶谱分析芽枝霉菌Lcxs9可以产2种β-葡萄糖苷酶。该文首次报道芽枝霉菌产高活性β-葡萄糖苷酶。A fungal Lcxs9 that produces high amount of β-glucosidase was isolated from rotten wood.Lcxs9 was identified as Cladosporium cladosporioides by morphological characteristics and ITS rDNA sequence analysis,with a yield of 7.18U/mL of β-glucosidase.The activity of β-glucosidase was stable within pH value 4~9 and temperatures below 60℃,respectively.Native-PAGE and activity staining showed that the extracellular enzyme contained two types of β-glucosidases.It was first reported isolated C.cladosporioides strain producing β-glucosidase with high activities.

关 键 词：Β-葡萄糖苷酶 筛选 鉴定 芽枝霉菌 酶学性质 

分 类 号：Q93-331[生物学—微生物学]