气相色谱柱前衍生化测定竹叶多糖超临界CO_2提取物  

Supercritical CO_2 Fluid Extraction and Pre-column Derivatization GC Analysis of Polysaccharides Bamboo Leaf Polysaccharides in a Static-dynamic Mode

作　　者：任美玲[1] 吕兆林[2] 欧阳呓林[1] 林西[1] 张柏林[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学分析测试中心,北京100083

出　　处：《食品科学》2012年第6期215-219,共5页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD19B08);国家林业公益性行业科研专项(200904014)

摘　　要：对毛竹(Phyllostachys heterocycla)、苦竹(Pleioblastus amarus)、绿竹(Dendrocalamopsis oldhami)及黄甜竹(Acidosasa edulis)4个种的竹子竹叶多糖进行研究。采用超临界CO2流体高效萃取竹叶多糖,以体积分数为75%的乙醇溶液为夹带剂,萃取釜温度50℃,萃取压力40MPa,夹带剂用量与竹叶的质量的比为3:1(mL/g),采用静态萃取与动态萃取兼顾的提取方式,萃取时间各1h,其中,绿竹叶(均以干叶计算)中多糖含量最高(16.585mg/g),其次是黄甜竹(12.804mg/g)、毛竹(8.255mg/g)、苦竹(6.329mg/g)。并对4个种的竹叶多糖经三氟乙酸水解、乙酰化和气相色谱分析,研究其单糖的物化特征,结果表明,4个种的竹叶多糖中均含甘露糖、鼠李糖、半乳糖、葡萄糖、阿拉伯糖、木糖和岩藻糖,毛竹、绿竹、苦竹叶中葡萄糖含量较高,黄甜竹叶中半乳糖较高,绿竹叶中的葡萄糖含量可达2.345mg/g,半乳糖含量可达1.432mg/g,这两种单糖的含量在4个种的竹叶中最高。Static-dynamic supercritical CO2 fluid extraction was carried out at 50 ℃ and 40 MPa for 1 h in each mode using 75% ethanol as the entrainer for extracting Bamboo polysaccharides from the leaves of Phyllostachys heterocycla,Pleioblastus amarus,Dendrocalamopsis oldhami,and Acidosasa edulis at an entrainer-to-material ratio of 3：1（mL/g）.The polysaccharide contents of the leaves of Phyllostachys heterocycla,Pleioblastus amarus,Dendrocalamopsis oldhami,and Acidosasa edulis,as calculated based on a dry matter basis,were 16.585,12.804,8.255 mg/g and 6.329 mg/g,respectively.The polysaccharide samples obtained were hydrolyzed with trifluoroacetic（TFA）,acetylated and analyzed for monosaccharide composition by gas chromatography.Each polysaccharide contained mannose,rhamnose,galactose,glucose,arabinose,xylose and fucose.The predominant saccharide was glucose in D.oldhami,P.amarus and P.heterocycla and galactose in A.edulism.D.oldhami leaves contained 2.345 mg/g glucose and 1.432 mg/g and galactose,both of which were the most predominant monosaccharides in the four species of bamboo leaves.

关 键 词：超临界CO2流体萃取 竹叶多糖 气相色谱 柱前衍生化 单糖特征 

分 类 号：TS201.4[轻工技术与工程—食品科学]