厚壳贻贝血细胞颗粒的蛋白质组学分析  

Proteomic analysis of granule of hemocytes from Mytilus coruscus

作　　者：王信超[1] 孙敬敬[1] 范美华[1] 武梅[1] 石戈[1] 王日昕[1] 廖智[1] 

机构地区：[1]浙江海洋学院海洋科学学院,海洋生物资源及分子工程实验室,浙江舟山316004

出　　处：《南方水产科学》2012年第2期7-14,共8页South China Fisheries Science

基　　金：浙江省科技厅面上科研农业项目(2008C22026;2009C32016)

摘　　要：贝类的血细胞在宿主的免疫防御机制中发挥着重要的作用。文章利用线性离子阱-四级杆质谱对厚壳贻贝(Mytilus coruscus)血细胞的颗粒蛋白质组分进行了Shotgun分析,结合Mascot数据库搜索,共鉴定了125种高丰度蛋白,其中包括细胞骨架蛋白、细胞粘附蛋白、精氨酸激酶和代谢相关酶类等。此结果为深入了解厚壳贻贝血细胞颗粒的蛋白组成、贻贝免疫分子标记的筛选及其免疫机制奠定了基础。The blood cells of mussel play an important role in the host＇s immune defense mechanism. We explore the protein composi- tion in the granule of hemocytes from Mytilus coruscus by using liuear-ion-trap-quadrupole mass spectrometry （LTQ-Orbitrap） combined with Shotgun strategy. A total of 125 high-abundance proteins are identified by Mascot searching methods, including cytoskeletal pro- teins, cell adhesion proteins, arginine kinases and metabolism-related enzymes, etc.. The results lay a foundation for further studies on the composition of mussel protein particles, screening of its immune molecular markers as well as its immune mechanism.

关 键 词：厚壳贻贝 血细胞颗粒 LTQ—Orbitrap 质谱 SHOTGUN 

分 类 号：S917.4[农业科学—水产科学]