5个不同地区绞股蓝中挥发性成分的SPME-GC-MS分析  

SPME-GC-MS analysis on volatile components from five different regions of Gynostemma pentaphyllum(Thunb.)

作　　者：牛俊峰[1] 肖娅萍[1] 姜东亮[1] 王璐[1] 吕鼎豪[1] 李封辰[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,西安710062

出　　处：《药物分析杂志》2012年第4期578-582,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家"十二五"科技支撑计划项目(2011BA106B05)

摘　　要：目的:建立固相微萃取-气相色谱质谱方法,对采自4省市5个地区药用植物绞股蓝的挥发性化学成分进行定性定量分析。方法:采用固相微萃取-气相色谱-质谱(SPME-GC-MS)联用技术对绞股蓝挥发性成分进行分析,利用峰面积归一化法测定各个成分的相对百分含量。结果:5个地区的绞股蓝中,总共分离出67个成分,主要含有醛类、酮类、烷烃类、烯烃类、芳香烃类、醇类和脂类。5个样品中共有成分6个,分别为:3,7-二甲基-1,6-辛二烯-3-醇、萘、α-紫罗酮、香叶基丙酮、β-紫罗酮和6,10,14-三甲基-2-十五烷酮。其中平利绞股蓝中含量最高的成分为3-辛酮(14.93%),宁陕鱼洞村绞股蓝中含量最高的为香叶基丙酮(10.8%),湖南麻姑仙境绞股蓝中含量最高的为3,7-二甲基-1,6-辛二烯-3-醇(22.4%),重庆缙云山和四川青城山绞股蓝中含量最高的均为安息香醛,含量分别为63.16%和38.52%。结论:5个地区绞股蓝中挥发性成分差异性很大。Objective：To establish a SPME-GC-MS method for qualitative and quantitative analysis of the essential oil composition of Gynostemma pentaphyllum in five areas of four provinces.Methods：The essential oil were analysed by SPME-GC-MS,and the contents were calculated with normalization method.Results：There were 67 compositions of Gynostemma pentaphyllum in five areas.The main constituents were aldehyde,ketone,alkane,alkene,aromatic hydrocarbons,alcohol and ester compounds.There were six common compositions of five samples,which were（3,7-dimethyl-1,6-octadien-3-ol）,naphthalene,[（E）-4-（2,6,6-trimethyl-2-cyclohexen-1-yl）-3-buten-2-one],[（E）-6,10-dimethyl-5,9-undecadien-2-one],[（E）-4-（2,6,6-trimethyl-1-cyclohexen-1-yl）-3-buten-2-one] and（6,10,14-trimethyl-2-pentadecanone）.The highest content of Pinglixian（abbr.PL） sample was 3-octanone which reached 14.93%.The highest content of Yudongcun（abbr.YDC） was [（E）-6,10-dimethyl-5,9-undecadien-2-one] and reached 10.8%,in the sample of Maguxianjing（abbr.MGXJ）,the highest one was（3,7-dimethyl-1,6-octadien-3-ol） and reached 22.4%,and the highest content of Jinyunshan（abbr.JYS） and Qingchengshan（abbr.QCS） was benzaldehyde,63.16% and 38.52% respectively.Conclusion：The volatile oil between different areas of Gynostemma pentaphyllum was significant difference.

关 键 词：绞股蓝 挥发性成分 固相微萃取 气质联用 

分 类 号：R917[医药卫生—药物分析学]