分光光度法测定菊叶薯蓣中薯蓣总皂甙含量的研究  

The Study on Determination of Dioscin Content by UV Spectrophotometry

作　　者：沈晖[1] 赵丛丛[1] 刘曾[1] 曾凡骏[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065

出　　处：《食品与发酵科技》2012年第2期104-106,共3页Food and Fermentation Science & Technology

摘　　要：采用紫外分光光度计测定菊叶薯蓣中薯蓣总皂甙含量,确定薯蓣总皂甙的最大吸收波长为480nm,最佳实验条件为:5%香草醛-冰醋酸溶液的加量0.2mL,高氯酸溶液的加量0.8mL,水浴温度70℃,水浴时间15min。精密度实验的相对标准偏差(RSD)为1.25%,加标回收率实验的平均回收率为98.87%。该方法具有操作快捷、精密度高、重现性好等优点。By UV spectrophotometry the maximum wavelength of dioscin was determined at 480nm,and the optimized experiment conditions were studied： 5% vanillin-glacial acetic acid solution dosage of 0.2ml,high acid solution dosage of 0.8mL,water bath temperature of 70℃,water bath time of 15min.The RSD of precision experiment was 1.12%,and the average recovery of experiment adding standard could be 98.87%.The method was simple,sensitive,and reproduced well.

关 键 词：紫外分光法 薯蓣总皂甙 菊叶薯蓣 

分 类 号：TS207[轻工技术与工程—食品科学] Q155.5[轻工技术与工程—食品科学与工程]