液质联用法测定鲜蛋黄中的棉酚  

Determination of Gossypol in Egg Yolk by HPLC-MS

作　　者：施琦贻[1] 

机构地区：[1]上海市食品研究所,上海200235

出　　处：《现代食品科技》2012年第5期598-600,共3页Modern Food Science and Technology

摘　　要：采用HPLC-ESI-MS/MS联用技术,检测鲜蛋黄中的棉酚残留。样品经丙酮提取后,以C18柱为液相分离柱,以甲醇和1%甲酸为流动相,多反应监测模式测定棉酚,定性定量离子对分别为517.4/259.3、517.4/231.3。棉酚含量在1 ng/mL^100 ng/mL范围内具有良好的线性关系,测定下限(10 S/N)为1 ng/mL。以鲜蛋黄为基体,加入3种不同浓度水平的棉酚做加标回收试验,回收率在85%~100%之间,相对标准偏差(n=6)小于10%。该方法操作简单,结果准确,重现性好。Determination of gossypol in egg yolk samples was studied by using HPLC-ESI-MS/MS. The sample was extracted with acetone and analyzed on a C18 column using methanol and 1% formic acid as mobile phase. The monitoring ions of gossypol were 517.4/259.3 and 517.4/231.3 in the multiple reaction monitoring mode （MRM）. Linear relationship between values of peak area and mass concentration of gossypol was obtained in the range of 1 ng/mL-100 ng/mL, with lower limit detection （10 S/N） of 1 ng/mL. Recovery test was made by addition of standard gossypol with different concentrations. The recovery was found in the range of 85%-100%. RSD was found to be less than 10% （n=6）. The method was simple, sensitive, accurate and repeatable.

关 键 词：液质联用 蛋黄 棉酚 

分 类 号：R155.5[医药卫生—营养与食品卫生学]