N^+低能离子注入与亚硝酸钠复合诱变筛选子囊霉素高产菌株的研究  

Screening of high ascomycin-producing strains by N^+ ion implantation and NaNO_2 complex mutation

作　　者：王瑶函[1] 胡永红[1] 杨文革[1] 唐荣荣[1] 孟中斌[1] 沈飞 李栋芸 于辉 冯旌 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009 [2]南京新百药业有限公司,江苏南京210038

出　　处：《中国酿造》2012年第4期137-140,共4页China Brewing

基　　金：江苏省"六大人才高峰"项目(NO 2009118);江苏省产学研联合创新资金--前瞻性联合研究(BY2011106);南京市科技发展计划项目(2010ZD122)

摘　　要：为了获得子囊霉素高产菌株,以链霉菌NJWGH1322为研究对象,利用N+低能离子注入、亚硝酸钠(NaNO2)进行诱变。试验确定了最佳诱变条件:离子注入诱变剂量为200×1014ions/cm2;NaNO2诱变时间为5min。之后通过结合2种诱变方式进行复合诱变,结果显示:复合诱变效果明显优于单因子诱变。试验最终筛选出1株产量突变提高43.1%的高产菌株,经5次传代试验表明该菌株遗传稳定性较好。In order to obtain high ascomycin-producing strains,the strain NJWGH1322 was used as original strain for mutation by N＋ ion implantation and NaNO2.The optimal amount of ion beam was 200×1014 ions/cm2 and the mutation time of NaNO2 was 5min.The both method were combined to do complex mutation.The results showed that the effect of complex mutation was much better than that of single mutation.A high ascomycin-producing strain was obtained using complex mutation.The production of ascomycin was increased by 43.1% compared to that of the original strain.The strain showed good stability after five generations.

关 键 词：子囊霉素 N+低能离子注入 亚硝酸钠 复合诱变 

分 类 号：TQ929[轻工技术与工程—发酵工程]