离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖  

Determination of Glucose,Sucrose and Lactose in Milk Powder by Ion Chromatography

作　　者：熊建飞[1] 周光明[1] 许丽[1] 张磊[1] 

机构地区：[1]发光与实时分析教育部重点实验室,西南大学化学化工学院,重庆400715

出　　处：《食品科学》2012年第8期176-179,共4页Food Science

基　　金：重庆市自然科学基金项目(CSTC;2007BB5370);国家科技部重大专项(2008ZX07315)

摘　　要：建立高效阴离子交换色谱-脉冲安培检测法测定奶粉中葡萄糖、蔗糖和乳糖的方法。以METROSEP CARB1(150mm×4.0mm)阴离子交换柱为分离柱,脉冲安培检测,37mmol/L NaOH溶液为淋洗液,以柠檬酸作为蛋白质沉淀剂。葡萄糖、蔗糖和乳糖的线性范围分别为1～40、1～50、1～50mg/L,相关系数分别0.9966、0.9968、0.9985,检出限分别为0.014、0.091、0.083mg/L,精密度为1.10%～4.96%,回收率为90.13%～104.83%。该方法分析时间短,前处理简单,适用于快速测定奶粉中的葡萄糖、蔗糖和乳糖。A high performance anion exchange chromatographic-pulse amperometric detection method was established to determine glucose,sucrose and lactose in milk powder.The separation column used was METROSEP CARB 1（150 mm × 4.0 mm） anion exchange column,and the mobile phase was 37 mmol/L NaOH.Citric acid was used to precipitate proteins from milk.The linear ranges of glucose,sucrose and lactose were 1-40,1-50 mg/L and 1-50 mg/L with correlation coefficients of 0.9966,0.9968 and 0.9985,respectively,and the detection limits were 0.014,0.091 mg/L and 0.083 mg/L.The precision relative standard deviations of precision for 5 replicate determinations were in the range of 1.10%-4.96%.The recovery rates for glucose,sucrose and lactose in 4 different commercial samples were 90.13%-104.83%.The method was suitable for rapid determination of glucose,sucrose and lactose in milk powder,thanks to its short analysis time and simple pretreatment.

关 键 词：高效阴离子交换色谱 脉冲安培检测 奶粉 葡萄糖 蔗糖 乳糖 柠檬酸 

分 类 号：O657.75[理学—分析化学]