不同培养条件和前体对蛹虫草液体发酵产虫草素的影响  

Effects of additives and different culture conditions on cordycepin production by the medicinal fungus Cordyceps militaris

作　　者：康超[1,2] 文庭池[1] 康冀川[1] 钱一鑫[1] 雷帮星[1] 

机构地区：[1]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《菌物学报》2012年第3期389-397,共9页Mycosystema

基　　金：贵州省教育厅自然科学研究项目[黔教科(2009)0129号];贵州省"十二五"农业科技攻关项目[黔科合NY字(2011)3054号]

摘　　要：蛹虫草能产生虫草素等多种活性物质。为考察不同液体发酵方式及添加前体物质对虫草素积累的影响,选用蛹虫草08Y1菌株,通过光照振荡、光照静置、黑暗振荡、黑暗静置四种培养条件和添加前体物质(腺嘌呤1g/L+甘氨酸16g/L),发酵16d后检测虫草素和腺嘌呤含量。结果表明:08Y1菌株在黑暗振荡培养条件下,虫草素积累达1,015.0mg/L,腺嘌呤利用率达98.5%,说明黑暗振荡培养并添加前体物质是提高虫草素产量的有效方法。The medicinal fungus Cordyceps militaris produces many active metabolites including cordycepin （3＇-deoxyadenosine）. The effects of additives and different liquid culture conditions on cordycepin production by C. militaris were investigated. The strain of C. militaris 08Y1 was used for optimizing liquid culture conditions （light and shaking, light and static, dark and shaking, dark and static）, and the additives （adenine 1 g/L＋glycine 16g/L） were added to medium for 16 days. The maximum cordycepin production of 1,015.0mg/L was achieved by culture at dark and shaking with the supplement of additives, and the maximum utilization ratio of adenine reached 98.5%. The results showed that the condition of dark and shaking with the supplement of additives was an effective way for increasing the production of cordycepin.

关 键 词：前体物质 液体发酵 培养条件 虫草素 蛹虫草 振荡培养 活性物质 发酵方式 

分 类 号：S567.35[农业科学—中草药栽培]