浒苔多糖脱蛋白脱盐方法的优选  

Laboratory Experiment Optimization of Desalting and De-protein Process for Enteromorpha Polysaccharides

作　　者：陈达妙[1] 林文庭[1] 

机构地区：[1]福建医科大学公共卫生学院营养与保健医学系,福州350004

出　　处：《中国食物与营养》2012年第4期59-63,共5页Food and Nutrition in China

基　　金：福建省科技计划项目(项目编号:2011N0015)

摘　　要：目的:探讨浒苔多糖脱蛋白脱盐的方法。方法:利用蛋白酶水解浒苔多糖的蛋白质,通过正交试验优选蛋白酶水解工艺条件;利用透析袋流水透析脱盐,通过正交试验优选透析工艺条件。结果:中性蛋白酶脱蛋白效果优于Sevag法和三氯乙酸法,中性蛋白酶脱蛋白较佳工艺条件为:加酶量0.12g/10mL、pH6.0、反应温度45℃、反应时间1h,在此条件下,蛋白质脱除率达52.36%。透析脱盐的较佳条件为:7000Da、透析时间8h、浒苔多糖溶液浓度6%,在此条件下,灰分脱除率为76.95%。(除杂后,浒苔多糖蛋白质含量为3.43%,灰分含量为8.04%,多糖损失率为18.96%)。结论:蛋白酶法脱蛋白质技术和透析袋流水透析脱盐技术,对浒苔多糖的脱蛋白脱盐效果较好,有一定的推广潜力。【Objective】 To study the method of de-protein and desalting process for enteromorpha polysaccharides.【Method】The best method of enzymatic de-protein process was screened from amino-acid nitrogen.The proper method of run water dialysis was chosen from dry ash content and polysaccharides loss rate.【Result】De-protein effect of neutral protease was better than that of papain method,Sevag method and trichloroacetic acid method.The best condition of neutral protease de-protein process was adding 0.12g/10mL of lipase amounts into the extracting liquid,adjusting the pH to 6.0,then keeping it in the constant temperature bath 45℃ for 1h.The proper method of run water dialysis was keeping 6% enteromorpha extracting liquid in the 7？000Da dialysis bag for 8h.The protein content of purer enteromorpha polysaccharides was 3.43%,ash content was 8.04%,and polysaccharides loss rate was 18.96%.【Conclusion】 Neutral protease method and of run water dialysis method are the better ways for de-protein effect and desalting process,which can be used universally.

关 键 词：浒苔多糖 脱蛋白 脱盐 酶法 透析法 

分 类 号：TQ461[化学工程—制药化工]