响应面法优化Vc二步发酵新菌系产2-酮基-L-古龙酸的培养基  

Response Surface Methodology to Optimize Medium for 2-Keto-L-Gulonate Production by New Strains System in Two-Step Vitamin C Fermentation

作　　者：徐婷婷[1] 高明[1] 吕淑霞[1] 于晓丹[1] 张忠泽[2] 陈宏权 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [3]东北制药总厂Vc公司,辽宁沈阳110028

出　　处：《微生物学杂志》2012年第2期47-53,共7页Journal of Microbiology

基　　金：沈阳市科技计划项目(F10-205-1-55);沈阳农业大学NSFC启动基金资助项目(20102002)

摘　　要：以短小芽胞杆菌(Bacillus pumilus)HJ-04作为维生素C二步发酵第2步中的伴生菌,促进产酸菌产维生素C(Vitamin C,Vc)前体2-酮基-L-古龙酸(2-keto-L-gulonic acid,2-KGA)的能力强于工业生产用菌株巨大芽胞杆菌(Bacillus megaterium)B2980。采用单因素试验、Plackett-Burman(PB)试验及Box-Behnken试验对影响新菌系发酵产2-KGA的6个因素进行分析优化。结果表明,L-山梨糖、尿素、玉米浆为显著影响因子。最佳产酸条件为L-山梨糖94.95 g/L,尿素11.99 g/L,玉米浆14.13 g/L。优化后产酸量提高12.31 mg/mL,产酸周期缩短6 h。The ability of Bacillus pumilus HJ-04 to promote the production of vitamin C（VC） precursor,2-keto-L-gulonic acid（2-KGA）,by 2-KGA-producing strain is stronger than industrial Bacillus megaterium B2980 as associated bacteria in second step of two-step VC fermentation.Six factors that affected the 2-KGA production by new strains system were analyzed and optimized adopting single factor test,Plackett-Burman（PB） design,and Box-Behnken design.The results indicated that L-sorbose,urea,and corn syrup were the noticeably influencing factors.The optimum acid-producing condition were 94.95 g/L of L-sorbose,11.99 g/L of urea,14.13 g/L of corn syrup.The acid production increased by 12.31 mg/mL,and the production period shortened by 6 hours after the optimization.

关 键 词：维生素C二步发酵 2-酮基-L-古龙酸 短小芽胞杆菌 条件优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]