高温大曲中筛选产纤维素酶的耐高温芽孢杆菌  

Screening of a Thermostable Cellulase-secreting Bacillus from High Temperature Daqu

作　　者：龚丽琼[1,2,3] 邓朝霞[1,2,3] 黄祖新[1,2,3,4] 颜林春[1,2,3] 马校卫[1,2,3] 汤二将[1,2,3] 陈由强[1,2,3,4] 陈如凯[2] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108 [3]福建省发育与神经生物学重点实验室．福建福州350108 [4]教育部工业微生物工程研究中心,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2012年第3期106-112,共7页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家现代农业产业技术体系建设专项资金资助项目(CARS-20-4-4);福建省教育厅资助项目(JK2010013;03BC727)

摘　　要：从高温大曲中筛选出产纤维素酶的芽孢杆菌1株,进行形态特征、生化鉴定和16S rDNA序列分子鉴定,该菌株鉴定为Bacillus cereusFrankland.对该菌株进行产酶摇瓶液体发酵,测定其内切纤维素酶活力和外切纤维素酶活力分别为260.32,87.22u.mL-1,对该菌株进行产酶固态发酵,测定其内切纤维素酶活力和外切纤维素酶活力分别为556.64,121.47u.mL-1.酶的稳定性实验显示该菌株产纤维素酶在60℃以下和在pH4～8范围内具有良好的热稳定性和pH稳定性,利用响应面法优化固态发酵产酶培养基,优化后测定纤维素酶活提高至1 643.27u.mL-1.The Bacillus that can produce cellulase is isolated and purified from the high temperature Daqu, based on the analysis of 16SrDNA and some biochemical and physiologicalcharacteristics, this strain was identified as Bacillus cereus Frankland. The shake flask liquid fermentation was taken to determine the endo-cellulase activity （260.32 u · mL^-1） , and exo- cellulase activity （87.22 u · mL^-1） , the solid state fermentation was taken to determine theendo-cellulase activity （556.64 u · mL^-1） and exo-cellulase activity （121.47 u · mL^-1）. High heat and pH stability of cellulase activity are observed at temperature lower than 60℃ and pH4-8. After the response surface method optimization, the cellulase activity of solid state fermentation increased to 1643.27 u · mL^-1.

关 键 词：大曲 纤维素酶 芽胞杆菌 液体发酵 固态发酵 响应面优化 

分 类 号：Q939.9[生物学—微生物学]