航天诱变菌株黑曲霉ZM-8发酵玉米秸秆产β-葡萄糖苷酶的条件优化及其酶学特性  

Optium condition of β-glycosidase produced by the strain Aspergillus niger ZM-8 screened by space mutation with corn stalk and bran as materials

作　　者：马旭光[1] 张宗舟[1] 柳芸[1] 赵国婵[1] 霍建泰 

机构地区：[1]天水师范学院生命科学与化学学院 [2]甘肃省航天育种工程技术研究中心

出　　处：《中国饲料》2012年第10期14-17,共4页China Feed

基　　金："十一五"国家科技支撑计划项目"西北内陆灌区农田循环生产技术集成研究与示范"(2007BAD89B17);甘肃省自然科学研究基金资助项目"航天诱变高产纤维素酶菌株黑曲霉的选育及其产酶条件的研究"(1010RJZE029);甘肃省教育厅科研项目"航天诱变菌株黑曲霉ZM-8β-葡萄糖苷酶的纯化及酶学性质的研究"(1108B-02)

摘　　要：黑曲霉ZM-8是一株经航天诱变筛选出的高产纤维素酶菌株。以玉米秸秆和麸皮为原料,利用固态发酵法研究了发酵时间、发酵温度和初始pH对该菌株产β-葡萄糖苷酶的影响,采用正交试验对各因素进行了优化,并对酶学特性进行了初步研究。结果表明,发酵时间和发酵温度对酶活影响均达到极显著水平(F=14.994>F0.01=5.85;F=10.872),初始pH(F=5.843)对其影响达到显著水平;当初始pH为5.5、培养温度为35℃、培养时间为72 h时,β-葡萄糖苷酶的酶活达到了58.95 U/mL。该酶最适温度为55℃,保温2 h后仍具有95%以上的酶活力;最适pH为5.5,pH在3.0～6.0时,酶活力稳定性良好。The strain Aspergillus niger ZM-8 with high cellulase activity was screened by space mutation with the corn stalk and bran as raw materials, the effects of fermentation time,fermentation temperature and initial pH on the β- glycosidase activity were studied by the method of solid-state fermentation and these factors were optimized by the orthog- onal experiment, and the enzymatic characteristics were determined in this paper.The results showed that： the effects of fer- mentation time（F=14.994）and fermentation temperature （F=10.872） on the β- glycosidase activity were extremely signifi- cant, the effect of initial pH was significantly （F=5.843〉Fo.=3.49）.The β-glycosidase activity reached 58.95 U/mL under the conditions of initial pH was 5.5,fermentation temperature was 35 ℃ and fermentation time was 72 h.The β-glycosi- dase optimun temperature was 55℃ and the residual enzymatic activity was more than 95 % after being kept for 2 h.lts optimun pH was 5.5 and the enzyme activity was stable from pH 3.0 to 6.0.

关 键 词：航天诱变 黑曲霉ZM-8 Β-葡萄糖苷酶 发酵条件 酶学特性 

分 类 号：S816.6[农业科学—饲料科学]