离子液体萃取/HPLC测定水产品中司帕沙星残留研究  

Application of ionic liquid as extraction agent and determination of sparfloxacin in the aquatic products by HPLC

作　　者：万雄[1,2] 郁迪[1] 董洁莹[1] 孙俏洁[1] 盛寒玉[1] 杨晓露[1] 季龙娟[1] 金丽华[1] 

机构地区：[1]浙江海洋学院食品与药学学院,舟山316000 [2]佳木斯大学药学院,佳木斯154007

出　　处：《食品科技》2012年第5期303-306,共4页Food Science and Technology

基　　金：浙江省自然科学基金项目(Z3100533;Y2111223);浙江省科技厅公益计划项目(2011C22073);浙江省新苗计划项目(2010R411012)

摘　　要：建立了以离子液体([HMIm]BF4)为萃取剂,HPLC检测水产品中残留司帕沙星的方法,采用反相SpherisorbC18色谱柱(250mm×4mmi.d.,5μm),流动相为甲醇,流速:0.5mL/min,检测波长:298nm。在0.06～0.18mg/mL浓度范围内,方法线性良好(R2=0.9988),回收率为88.7%～93.0%,日内和日间的RSD介于2.1%～3.7%。方法准确可靠,重现性好,灵敏度高,适用于水产品中残留药物的检测。Determination of sparfloxacin in the aquatic products using [HMIm] BF4 as extraction agent coupled with HPLC was investigated.The column configuration consisted of Spherisorb C18 column（250 mm×4 mm i.d.,5 μm particle size）.The mobile phase was methanol at a flow rate 0.5 mL/min,the UV detector was set at 298 nm.calibration curve was linear over the concentration ranges tested with 0.06～0.18 mg/mL（R2=0.9988）.The precision（RSD%） and accuracy were 2.1～3.7 and 88.7%～93.0%,respectively.The method was validated for linearity,precision and intra-and inter-day variation,and was suitable to the determination of drug residues in aquatic products.

关 键 词：离子液体 高效液相色谱 水产品 司帕沙星 

分 类 号：TS207.53[轻工技术与工程—食品科学]