环介导等温扩增技术快速检测奶粉中的单增李斯特菌  

Rapid Detection of Listeria monocytogenes in Milk Powder by Loop-mediated Isothermal Amplification

作　　者：路彦霞[1] 孟兆祥[2] 马晓燕[1] 王羽[1] 张先舟[1] 张伟[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]中国检验检疫科学研究院食品风险管理与应用研究所,北京100123

出　　处：《现代食品科技》2012年第6期703-706,共4页Modern Food Science and Technology

基　　金：河北省自然科学基金(C2008000216)

摘　　要：环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP)是一种在等温条件下特异、灵敏、快速的新型基因扩增技术。试验以单增李斯特菌为研究对象,根据其特有的hlyA基因设计了一套特异性引物,进行LAMP扩增。结果表明,LAMP检测单增李斯特菌的灵敏度为2.45×101cfu/mL,其对人工感染奶粉样品的检出限为7.32×101cfu/mL。对其它食品病原菌进行检测,结果均未出现目的条带,特异性强。表明LAMP法适合于食品中污染单增李斯特菌的快速检测。Loop-mediated isothermal amplification （LAMP） is a novel DNA amplification technology with high specificity, efficiency and rapidity under isothermal conditions. In this research, a set of specific LAMP primers were designed to amplify the specific hlyA gene of Listeria monocytogenes by LAMP technology. The results showed that the detection sensitivity and detection limit of the LAMP method for purely cultured Listeria monocytogenes were 2.45× 101 cfu/mL and 7.32× 101cfu/mL, respectively. For other food pathogens, the target bands were not found. This method was suitable for rapid detection ofListeria monocytogenes in contaminated foods.

关 键 词：单增李斯特菌 LAMP hlyA基因 快速检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]