枯草芽孢杆菌C3产抗菌物质发酵培养基的优化  

Optimization of fermentation medium for antimicrobial substances from Bacillus subtilis C3

作　　者：马晓丹[1,2] 张红星[1,2] 钟思琼[3] 谢远红[1,2] 刘慧[1,2] 

机构地区：[1]北京农学院食品科学与工程学院,北京102206 [2]农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206 [3]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《中国酿造》2012年第5期10-14,共5页China Brewing

基　　金："十二五"国家高技术研究发展计划(863)项目子课题(2012AA101606-05);国家"十二五"科技支撑计划项目(2012BAD28B00)

摘　　要：该文利用对黑曲霉孢子萌发有抑制作用的枯草芽孢杆菌C3,研究优化产抗菌物质的发酵培养基组分。在碳源、氮源、C/N单因素多水平试验结果的基础上,设计Na+、Mg2+、K+浓度3因素3水平L(933)正交试验,研究无机盐离子对枯草芽孢杆菌产抗菌物质的影响。结果表明,碳源为1%葡萄糖,氮源为蛋白胨和酵母浸粉各1.5%,碳氮比为1∶3,NaCl为0.5%,KH2PO4为0.1%,MgSO4.7H2O为0.15%时,枯草芽孢杆菌产抗菌物质的抑菌效果明显,对霉菌孢子萌发的抑制率提高了118.64%。Using Aspergillus niger spore germination of an inhibition of Bacillus subtilis C3,this paper optimizes antifungal substances of fermentation components for research.Based on the test results of carbon source,nitrogen source,C/N single factor with many levels,we design three factors three levels L9（33） orthogonal test of Na＋,Mg2＋,K＋ concentration to test the influence of inorganic salt ion to Bacillus subtilis antibacterial material.The results show that when the carbon source is 1% glucose,nitrogen source is peptone and yeast extract 1.5% each,C/N is 1 ∶ 3,NaCl is 0.5%,KH2PO4 is 0.1%,MgSO4·7H2O is 0.15%,the antibacterial effect from Bacillus subtilis antibacterial substance is obvious and the inhibition rate of fungus spore germination increases 118.64%.

关 键 词：枯草芽孢杆菌 抗菌物质 培养基优化 

分 类 号：Q93-335[生物学—微生物学]