溶氧状况对黑曲霉DB056产柚苷酶的影响研究  

Effects of oxygen conditions on naringinase production in Aspergillus niger DB056 fermentation

作　　者：张其标[1,2] 肖安风[1,3,2] 倪辉[1,3,2] 游洪燕[1] 蔡慧农[1,3,2] 

机构地区：[1]集美大学生物工程学院,厦门361021 [2]厦门市食品生物工程技术研究中心,厦门361021 [3]福建省高校食品微生物与酶工程研究中心,厦门361021

出　　处：《食品科技》2012年第6期6-10,共5页Food Science and Technology

基　　金：福建省科技计划重点项目(2009N0044);福建省科技计划重点项目(2011N0101);福建省自然科学基金项目(2011J01225);科技人员服务企业行动项目(2009GJC40050);集美大学中青年创新团队专项基金项目(2010A006)

摘　　要：以α-L-鼠李糖苷酶和柚苷酶的酶活为评价指标,从装液量、玻璃珠数量、氧载体这3个方面来研究黑曲霉DB056摇瓶发酵产柚苷酶的溶氧条件。通过单因素优化得到的摇瓶发酵条件为:装液量35mL、玻璃珠添加个数为3个(直径4mm)、最适氧载体为1%正己烷,在此条件下α-鼠李糖苷酶活力最大可达787.8U/mL,柚苷酶活力最大可达232.5U/mL。Naringinase is widely used in food, medicine, and so on. In this study, the dissolved oxygen condition in Aspergilius niger DB056 fermentation was researched by singer factor experiment. The optimum fermentation condition for enzyme production was 35 mL medium in 250 mL flask, adding three glass beads （diameter 4 mm） and adding 1% n-hexane in medium. Under this condition, α-L-rhamnosidase activity and naringinase activity detected by high performance liquid chromatography method were 787.8 U/mL and 232.5 U/mL, respectively.

关 键 词：黑曲霉 柚苷酶 氧载体 α-L-鼠李糖苷酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]