分子马达传感器对沙门氏菌快速检测方法的初步研究  

Preliminary study of a rapid detecting technology for Salmonella based on molecular motor biosensor

作　　者：张捷[1] 顾德周[1] 张惠媛[1] 杨泽慧 王佩荣[3] 张雷[1] 齐玮[1] 陈广全[1] 乐加昌[3] 

机构地区：[1]北京出入境检验检疫局,北京100026 [2]国家认监委认证认可技术研究所,北京100020 [3]中国科学院生物物理研究所,北京100101

出　　处：《食品工业科技》2012年第12期93-96,共4页Science and Technology of Food Industry

基　　金：国家质检总局科技计划项目(2008IK161)

摘　　要：利用F0F1-ATP合酶的旋转特性及对H+敏感的荧光物质F-DHPE作为指示剂来检测样本中有无目标物。通过生物素-亲和素系统将特异性invA核酸探针连接在F0F1-ATPase的ε亚基上构建生物传感器;将待测样品和阴性对照分别与生物传感器结合后,比较其催化ATP合成30min后的ATP产生量,依此对样品中的沙门氏菌DNA进行检测。结果表明,该方法对沙门氏菌DNA的检测时间为1h,检出限为10ng/mL。从食品样本分离得到的30株细菌,利用分子马达生物传感器的检测结果与PCR检测的结果一致。Using F0F1-ATPase of spin characteristics and H＋-sensitive fluorescent material F-DHPE as an indicator to detect the target sample.Specific invA probe were connected with F0F1-ATPase’s ε subunit by using avidin-biotin system,and then biosensors were constructed,the test sample and negative sample,respectively,combined with biosensors,to compare their catalytic ATP synthesis after 30min,Salmonella DNA in the samples can be tested.According to the result of our experiment,it took 1h and could detect accurately about 10ng/mL for standard strain.30 strains of food sample which were detected with the PCR detection results that were consistent.

关 键 词：沙门氏菌 F0F1-ATP合酶 分子马达传感器 快速检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]