豆豉纤溶酶高产突变株的选育  

Selection of High Yield Mutants of Douchi Fibrinolytic Enzyme

作　　者：黄晓杰[1] 矫明书[1] 吴晓萌[1] 

机构地区：[1]辽宁医学院食品科学与工程学院,辽宁锦州121001

出　　处：《安徽农业科学》2012年第17期9473-9474,共2页Journal of Anhui Agricultural Sciences

基　　金：辽宁医学院青年科技启动基金项目(Y2010Z027)

摘　　要：[目的]从豆豉中筛选出高产纤溶酶的菌株,并通过平板筛选得到高产突变株。[方法]从6种不同豆豉样品中,筛选得到具有较高纤溶酶活性的菌株,采用物理化学诱变方法进行复合诱变,再利用纤维蛋白平板对诱变后菌株进行筛选。[结果]得到1株高纤溶酶活力菌株,命名为DC-YC41,酶活达到742 IU/ml。[结论]初步判断,得到的这株高纤溶酶活力菌株为枯草芽孢杆菌。[ Objective ] To select the high yleld strains producing fibrinolytic enzyme from Douchi products and then find out the high-yield mu- tants through plate screening. [ Method ] The high yield strains producing fibrinolytic enzyme were screened out from six different Douchi samples, and then compound mutated by the physio-chemical mutation method, the mutants were screened out with fibrin plate. [ Result ] One high yield strain producing fibrinolytic enzyme was obtained and named DC-YC41, its enzyme activity was 742 IU/ml. [ Conclusion] The strain was prelimi- narily identified to be Bacillus subtilis.

关 键 词：豆豉纤溶酶 酶活 选育 

分 类 号：S188[农业科学—农业基础科学]