以琥珀酸为唯一碳源选育ε-聚赖氨酸高产菌  

Breeding of ε-poly-L-lysine producing strain using succinic acid as sole carbon source

作　　者：李凤[1] 李树[1] 任喜东[1] 王靓[1] 徐健[1] 毛忠贵[1] 唐蕾[1] 

机构地区：[1]江南大学 工业生物技术教育部重点实验室,无锡214122

出　　处：《工业微生物》2012年第3期50-54,共5页Industrial Microbiology

基　　金：无锡市科技支撑项目(CYE21N1107)

摘　　要：本文旨在通过诱变和耐受性筛选的手段提高放线菌ε-PL的产量。以稠李链霉菌Streptomyces padanus LS-L5为出发菌,经紫外诱变,在以琥珀酸为唯一碳源的培养基上筛选快速生长的菌株,测定突变株的ε-PL发酵水平和遗传稳定性。经初筛和复筛,获得突变株H3,其摇瓶发酵ε-PL产量为0.68 g/L,较出发菌提高了41%,传代4次产量基本稳定。H3在5 L发酵罐中分批发酵,ε-PL产量达到2.0 g/L,较出发菌提高了一倍。在固定二氧化碳的能力方面,突变株H3的PEP羧化酶酶活较出发菌株LS-L5的PEP羧化酶酶活提高了1.67倍。In order to improve the production ofε-poly-L-lysine by Streptamyces, the original strain, Streptomyces padanus LS-LS, was suffered from ultraviolet treatment and then selected on agar plate containing succinic acid as sole carbon source. The productivity and genetic stability of mutants were measured and among them the mutant H3 was selected. Its ε-PL output was 0.68 g/L by flask fermentation test, which was 41% higher than that of the original strain. In the 5 liter jar fermentation, the yield of ε-PL was 2.0 g/L, which was 2 fold of the wild type strain.

关 键 词：Ε-聚赖氨酸 稠李链霉菌 紫外诱变 琥珀酸 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]