蛋氨酸对体外培养奶牛乳腺上皮细胞泌乳能力的影响  

Effect of Methionine on Lactation Ability of Dairy Cow Mammary Epithelial Cells Cultured in vitro

作　　者：王佳丽[1] 高学军[1] 李庆章[1] 黄建国[1] 陆黎敏[1] 乔彬[1] 潘洪宝[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《乳业科学与技术》2012年第1期8-10,共3页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：国家"973"计划项目(2011CB100804)

摘　　要：研究蛋氨酸与乳蛋白合成及乳腺泌乳能力的关系。建立体外培养的奶牛乳腺上皮细胞模型,采用高效液相色谱法、实时荧光定量PCR、细胞活力分析仪、甘油三酯试剂盒检测不同浓度蛋氨酸(0、0.2、0.4、0.8、1.2、1.6mmol/L)对体外培养24h的奶牛乳腺上皮细胞泌乳的影响。结果表明,蛋氨酸在1.2mmol/L时对体外培养的奶牛乳腺上皮细胞增殖的促进作用最明显,乳糖分泌量最高,β-酪蛋白mRNA表达量最高且甘油三酯合成最多(P<0.01)。An in vitro model of dairy cow mammary epithelial cells （DCMECs） was developed and used to study the relationship between methionine and milk protein synthesis or the lactation ability of the mammary gland. After 24 hours of in vitro culture in the presence of various concentrations （0, 0.2, 0.4, 0.8, 1.2 mmol/L and 1.6 mmol/L） of methionine, the lactation ability of DCMECs was analyzed by high performance liquid Chromatography （HPLC）, real-time quantitative PCR （RT-PCR）, cell viability analyzer and triglyceride kit. It was found that methionine at 1.2 mmol/L had the most significant proliferation- promoting effect on DCMECs during the culture period. In addition, the highest amounts of lactose secretion, /3-casein mRNA expression and triglyceride synthesis were achieved.

关 键 词：蛋氨酸 奶牛乳腺上皮细胞 乳糖 Β-酪蛋白 甘油三酯 细胞增殖 

分 类 号：S823.5[农业科学—畜牧学]