降解黄曲霉毒素微生物筛选中降解与吸附结合作用的区分  

Distinguishing Degradation from Binding of AFB_1 in Screening of Aflatoxin-degrading Microorganisms

作　　者：李超波[1] 李文明[1] 杨文华[1] 杨小龙[1] 曹郁生[1] 

机构地区：[1]食品科学与技术国家重点实验室,南昌大学中德联合研究院,江西南昌330047

出　　处：《食品与发酵工业》2012年第5期17-21,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31060022)

摘　　要：黄曲霉毒素的污染不仅带来巨大的经济损失,而且严重危害人和动物的健康,因而生物降解真菌毒素一直引人关注。但在研究黄曲霉毒素微生物降解时,首先必须要有可靠的方法来判定毒素是被降解还是可逆的吸附结合。该文在黄曲霉毒素B1(AFB1)降解菌株筛选中,对区分降解与吸附结合作用的方法进行了研究,建立了可靠的分析方法,通过比较不同pH的影响,细胞灭活对AFB1去除的影响,分析菌株细胞水洗脱、有机溶剂萃取后AFB1的变化等方法,有效地排除了生物吸附结合以及pH的干扰,区分了降解作用和可逆的吸附作用,这些方法简单实用。据此,从动物粪便中成功筛选到了2株能高效降解AFB1的菌株F4、F7。Aflatoxin contamination not only leads to serious economic losses, but also is detrimental to human and animal health. Much attention has been paid to mycotoxins biodegradation in recent years. For investigation of microbial detoxification, however, a reliable method is needed to decide whether the toxin is degraded or reversibly bound. In this paper, several methods were tested to differentiate the degrading AFB1 from adsorbing during the screening of aflatoxin Bl-degrading microbes. Through comparing the AFB1 detoxification differences between living cells and dead cells, analyzing the residual AFB1 from microbial cells by aqueous washing and organic solvent extrac-ting, and maintaining a stable pH of medium, we have effectively excluded the biological adsorbing and binding and pH interferences, and distinguished the degradation from the reversible adsorbing. It has been demonstrated that F4 and F7 strains have high AFB1 degradation activity.

关 键 词：黄曲霉毒素B1 微生物降解 吸附结合 区分 

分 类 号：TQ927[轻工技术与工程—发酵工程]