DNA拷贝数变异与HBV相关肝细胞癌术后肝内复发的相关性研究  

Genomic analyses of DNA copy number alterations with intrahepatic recurrence in HBV-associated hepatocellular carcinoma after hepatectomy

作　　者：闻炳基[1] 朱忠政[1] 贺松琴[1] 胡柳燕[1] 董辉[2] 王爱忠[3] 丛文铭[2] 

机构地区：[1]解放军第一一三医院肿瘤科,浙江宁波315040 [2]第二军医大学东方肝胆外科医院病理科,200438 [3]中国人民解放军第一一三医院病理科,315040

出　　处：《临床肿瘤学杂志》2012年第6期481-484,共4页Chinese Clinical Oncology

基　　金：国家自然科学基金资助项目(30470791);南京军区医学科技创新基金(08MA023;);宁波市自然科学基金(2009A610126)

摘　　要：目的探讨利用DNA拷贝数变异(CNA)预测HBV相关肝细胞癌(HCC)术后肝内复发的可行性。方法采用高分辨率微阵列比较基因组杂交技术(aCGH),对47例HBV相关HCC肿瘤细胞基因组DNA的CNA情况进行检测;通过Kaplan-Meier生存分析和Cox风险比例模型,评估CNAs对HCC术后复发的影响。结果单因素生存分析结果显示,16q11.2-22.1是否丢失对HCC术后复发有显著影响(P=0.001),而基因组内其他CNAs均与复发无关。与16q11.2-22.1未丢失者相比,16q11.2-22.1丢失HCC患者的术后复发危险比(HR)为4.59(95%CI=1.64～12.84,P=0.004)。多因素Cox回归分析显示,16q11.2-22.1是否丢失是HCC术后复发的独立预测因素(HR=4.64,95%CI=1.65～13.06,P=0.004)。结论染色体片段16q11.2-22.1是否丢失是预测HBV相关HCC术后肝内复发的有效指标。Objective To investigate the prognostic value of DNA copy number alterations （CNAs） in predicting intrahepatic recurrence of HBV-associated hepatocellular carcinoma after hepatectomy. Methods The genomie CNA status in tumor tissue DNA was detected by high-resolution array comparative genomic hybridization （aCGH） in 47 HBV-associated HCC samples. The follow-up time of these patients was 3. 1-28. 0 months. The CNAs were analyzed for their association with intrahepatic recurrence by Kaplan- Meier and Cox proportional hazards models. Results Univariate analysis of the association between CNAs and intrahepatic recurrence revealed that 16ql 1.2-22. 1 loss was significantly associated with recurrence （ P = 0. 001 ）. HCC cases with 16ql 1.2-22. 1 loss as com- pared to those without the loss had a 4. 59-fold （95% CI = 1.64-12. 84 ,P = O. 004 ） increased hazard ratio （HR） for recurrence. Mult- ivariate analyses including 16ql 1.2-22. 1 loss and tumor stage showed that 16ql 1.2-22. 1 loss was an independent predictive factor for recurrence （HR =4. 64,95% CI = 1.65-13.06,P = 0. 004）. Conclusion These findings suggest that 16q11.2-22. 1 loss is associat- ed with intrahepatic recurrence of HBV-associated hepatocellular carcinoma after hepatectomy and may be used as a predictive marker.

关 键 词：微阵列比较基因组杂交 拷贝数变异 肝细胞癌 肝内复发 

分 类 号：R735.7[医药卫生—肿瘤]