醋酸纤维素/聚四氟乙烯复合膜固定化磷脂酶A_1的研究  

Study on phospholipase A_1 immobilized by CA/PTFE composite membrane

作　　者：时敏[1] 周晓丹[1] 李越[1] 李志平[1] 于殿宇[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2012年第13期127-130,134,共5页Science and Technology of Food Industry

基　　金：国家863高技术研究发展计划油脂非均相反应和多相分离精制技术(2010AA101503);国家十二五科技支撑计划大豆油加工关键技术和装备研究与示范(2011BAD02B01)

摘　　要：以醋酸纤维素/聚四氟乙烯复合膜为载体对磷脂酶进行吸附固定,以酶活力为指标,进行单因素实验,并采用响应面的方法进行分析,确定最佳的固定化条件为:酶液浓度0.06g/mL,吸附时间3h,温度60℃,搅拌速度200r/min,得到固定后的酶活力为4.0U/cm2。由SEM结果可知,CA/PTFE复合膜可较好的固定磷脂酶。The phospholipase was immobilized on the cellulose acetate （CA）/polytetrafluoroethylene （PTFE） by adsorption. The single-factor tests of immobilization condition were made though investigateing lipase activity, then the results were optimized by the response surface methodology. The optimal condition of the immobilization was as foilows：phospholipase concentration 0.06g/mL,adsorption for 3h,adsorption temperature at 60℃, oscillation velocity 200r/min,the activity of immobilized lipase reached 4.0U/cm2. From the results of SEM, phospholipase were well immobilized by the CA/PTFE.

关 键 词：醋酸纤维素/聚四氟乙烯复合膜 固定化 磷脂酶A1 响应面 SEM 

分 类 号：TS201.2[轻工技术与工程—食品科学]