产叶酸菌株的筛选及其发酵条件的优化  

Screening of folic acid producing strains and optimization of fermentation conditions

作　　者：徐玲燕[1] 潘秋月[2] 聂小华[1] 孟祥河[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014 [2]浙江经贸职业技术学院应用工程系,浙江杭州310018

出　　处：《食品工业科技》2012年第13期158-162,共5页Science and Technology of Food Industry

摘　　要：从土壤样本中筛选分离得到的菌株R-1和S-1具有产叶酸的能力,进行单因素实验和正交分析,优化碳源、氮源、初始pH、发酵时间等条件,叶酸产量提高约为10%;加入前体物质对氨基苯甲酸(PABA),菌株R-1和S-1产叶酸的能力显著提高,分别提高了2倍和1.8倍;最后进行正交分析得出各菌种的最佳发酵产叶酸的因素选择。结果显示,菌株R-1的最佳产叶酸条件是葡萄糖25g/L,柠檬酸铵4g/L,PABA0.3g/L,接种量2%,培养时间24h,初始pH6.5,叶酸产量达到了1.92μg/mL;菌株S-1最佳产叶酸条件是蔗糖20g/L,柠檬酸铵2g/L,PABA0.3g/L,接种量3%,培养时间48h,初始pH5.5,叶酸产量为1.31μg/mL。Strain R-1 and S-1 which can synthesize folic acid were isolated from soil sample. Single factor experiments and orthogonal experiments were conducted,and the optimization of fermentation conditions （carbon and nitrogen source,initial pH,fermentation time） could increase folic acid levels by approximately 10%. The addition of PABA resulted in about two-fold increase in Strain R-1 of folic acid yields,while the Strain S-1 increased 1.8-fold. The optimal fermentation conditions for strain R-1 were as follows：glucose 25g/L, ammonium citrate 4g/L,PABA 0.3g/L,inoculation volume of 2% （v/v）,initial pH 6.5,fermentation time 24h and the folic acid yield was up to 1.92μg/mL. Meanwhile,the folic acid yields by strain S-1 was 1.31μg/mL when fermentation was performed at sucrose 20g/L,ammonium citrate 2g/L,initial pH5.5 for 48h,with inoculation volume of 3%（v/v）,PABA dosa.qe of 0.3g/L.

关 键 词：叶酸 筛选 优化 对氨基苯甲酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]