PCR-DGGE用于浓香型大曲微生物群落分析的条件优化  

Optimization of Denaturing Gradient Gel Electrophoresis for Microbial Communities in Luzhou-flavor Daqu

作　　者：王世宽[1] 侯华 袁城金[1] 谢仁有[1] 洪玉程[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]四川江口醇酒业(集团)有限公司,四川巴中636400

出　　处：《四川理工学院学报（自然科学版）》2012年第3期5-8,共4页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：四川省科技厅自然科学基金项目(2008JY0091)

摘　　要：试验对浓香型大曲微生物群落的PCR-DGGE电泳最佳变性剂梯度范围、电泳时间进行了优化。结果表明:细菌DGGE电泳最佳变性剂浓度梯度为35%～55%;而真菌DGGE电泳最佳变性剂浓度梯度为30%～50%。通过时间进程法得出最佳电泳时间为16小时。运用此优化后的PCR-DGGE技术对不同时期曲药微生物群落进行了研究,获得了较丰富的微生物多样性。Ploymerase chain reaction and denaturing gradient gel electrophoresis （PCR-DGGE） technique is used to reveal the Microbial Communities in Luzhou-flavor Daqu. In order to improve the accuracy, sensitivity and repeatability of DGGE, the running parameters of DGGE are optimized by level electrophoresis and electrophoresis time. The results show that the best range of denaturing gradients is 35% ~ 55% of DGGE of bacteria; the best range of denaturing gradients is 30% ~ 50% of DGGE of fungi ; the best eleetrophoretic time is 16 hours through the time process method. The Daqu microbial com- munity of different periods is researched by the optimized PCR-DGGE technology,and rich microbial diversity is obtained.

关 键 词：浓香型大曲 微生物 PCR—DGGE 变性剂 

分 类 号：Q145[生物学—生态学]